非酒精性脂肪肝病中长链非编码RNA的表达谱

Expression profile of long non-coding RNA in non-alcoholic fatty liver disease

目的:分析非酒精性脂肪肝病(non-alcoholic fatty liver disease,NAFLD)中长链非编码RNA(lncRNA)的表达谱特征。方法:采用lncRNA-m RNA基因芯片检测单纯性胆囊结石伴或不伴NAFLD患者的肝组织样本,获得lncRNA差异性表达谱。进一步使用生物信息学技术对差异表达的基因予以分析。结果:与正常样本相比,NAFLD肝样本内共有1 735个lncRNAs和1 485个mRNAs异常表达,其中535个lncRNAs和760个mRNAs上调,1 200个lncRNAs和725个mRNAs下调。结论:与正常肝组织相比,NAFLD组织中lncRNA表达谱明显异常,这些lncRNAs可能在NAFLD的发生和发展中发挥重要作用。

Objective： To analyze the characteristics of long non-coding RNA （lncRNA） expression in non- alcoholic fatty liver disease （NAFLD）. Methods： IncRNA-mRNA microarray was conducted on the liver tissue samples from 10 patients with simple gallbladder stone （5 NAFLD liver samples and 5 normal liver samples）, and the differentially expressed lncRNA was analyzed by bioinformatics technology. Results： Compared with the normal liver samples, there were abnormal expression of 1 735 lncRNAs and 1 485 mRNAs in NAFLD liver samples. Among them, 535 lncRNAs and 760 mRNAs were up-regulated, 1 200 lncRNAs and 725 mRNAs were down-regulated. Conclusion： Compared with normal liver, the expression of lncRNA in NAFLD tissues is obviously abnormal. These lncRNAs may play an important role in the occurrence and development of NAFLD.