摘要
目的:分析非酒精性脂肪肝病(non-alcoholic fatty liver disease,NAFLD)中长链非编码RNA(lncRNA)的表达谱特征。方法:采用lncRNA-m RNA基因芯片检测单纯性胆囊结石伴或不伴NAFLD患者的肝组织样本,获得lncRNA差异性表达谱。进一步使用生物信息学技术对差异表达的基因予以分析。结果:与正常样本相比,NAFLD肝样本内共有1 735个lncRNAs和1 485个mRNAs异常表达,其中535个lncRNAs和760个mRNAs上调,1 200个lncRNAs和725个mRNAs下调。结论:与正常肝组织相比,NAFLD组织中lncRNA表达谱明显异常,这些lncRNAs可能在NAFLD的发生和发展中发挥重要作用。
Objective: To analyze the characteristics of long non-coding RNA (lncRNA) expression in non- alcoholic fatty liver disease (NAFLD). Methods: IncRNA-mRNA microarray was conducted on the liver tissue samples from 10 patients with simple gallbladder stone (5 NAFLD liver samples and 5 normal liver samples), and the differentially expressed lncRNA was analyzed by bioinformatics technology. Results: Compared with the normal liver samples, there were abnormal expression of 1 735 lncRNAs and 1 485 mRNAs in NAFLD liver samples. Among them, 535 lncRNAs and 760 mRNAs were up-regulated, 1 200 lncRNAs and 725 mRNAs were down-regulated. Conclusion: Compared with normal liver, the expression of lncRNA in NAFLD tissues is obviously abnormal. These lncRNAs may play an important role in the occurrence and development of NAFLD.
出处
《中南大学学报(医学版)》
CAS
CSCD
北大核心
2017年第7期741-748,共8页
Journal of Central South University :Medical Science
基金
国家自然科学基金(81600461)~~