抑制非酶糖基化食用菌的筛选及抑糖基化物质提取工艺优化

Screening of mushroom inhibiting non-enzymatic protein glycosylation(NEPG) and extracting process optimization of inhibiting NEPG substance

蛋白质非酶糖基化(non-enzymatic protein glycation,NEPG)是引起糖尿病并发症的主要因素之一,因此开发具有抑制非酶糖基化反应的天然活性物质对治疗糖尿病并发症有重大意义。本文以二甲双胍作为阳性对照,比较了灰树花Grifola frondosa、银耳Tremella fuciformis、黑木耳Auricularia heimuer、毛头鬼伞Coprinus comatus、猴头菇Hericium erinaceus、茯苓Wolfiporia cocos 6种食用菌水提物和醇提物在体外对蛋白质非酶糖基化反应抑制效果。结果表明,灰树花水提物对非酶糖基化反应有良好的抑制效果,食用菌醇提物对非酶糖基化没有抑制效果。利用二次正交旋转组合试验对抑制率最高的灰树花活性物质的提取条件进行了优化,确定了灰树花的最佳提取温度85℃,提取时间2.25h,料液比1:30,抑制率达到(96.28±0.16)%。灰树花水提物在预防糖尿病并发症方面有良好的应用前景。

The non-enzymatic protein glycosylation （NEPG） is one of the main factors causing diabetes complication, therefore it played a significant role to explore natural active substances inhibiting NEPG reaction. In this study, using metformin as positivecontrol, the inhibitory effects of alcohol extracts and water extracts from six mushrooms including Grifola frondosa, Tremella fuciformis, Auriculorio heimuer, Coprinus comatus, Hericium erinaceus and Wolfiporia cocos on NEPG in vitro were compared The experiment results showed that the water extract from G. frondosa held highest inhibitory activity on NEPG reaction. The alcohol extracts from six mushrooms could not inhibit NEPG reaction in vitro. The extracting conditions of G. frondoso active substances were optimized by quadratic rotation-orthogonal combination design. The optimal extracting temperature and duration for G. frondoso active substances were 85℃ and 2.25h, respectively. The optimal ratio of material to liquid was 1：30 Under these optimal extracting conditions, the inhibitory rate of the extract on NEPG reaction was （96.28±0.16）%, indicating that the water extract of G. frondosa had good prospects in the prevention of diabetes complication.