沙蒿籽总黄酮和总多酚及其抗氧化活性

Total flavonoids and total polyphenolic content,and its antioxidant ability of Artemisia sphaerocephala Krasch seeds

本文测定了沙蒿籽的四种不同极性溶剂提取物中总黄酮和总多酚的含量、化学成分及其体外抗氧化活性。结果显示:沙蒿籽总黄酮和总多酚的提取含量与溶剂的极性相关,甲醇提取物的总黄酮(33.30±0.11)mg/g和总多酚(80.1±0.16)mg/g含量最高,其次是丙酮和乙酸乙酯提取物,氯仿提取物含量较低。相对应的是,甲醇提取物的DPPH和ABTS自由基清除能力最强,其IC50分别是19.09、26.30μg/m L,其铁离子还原能力也明显高于其它三种提取物。通过HPLC法检出槲皮素和儿茶素两种黄酮,均在甲醇提取物中含量最高,分别为5.15、28.24 mg/g。这表明,甲醇更适宜于沙蒿籽中总多酚和总黄酮的提取,这为沙蒿籽作为抗氧剂的开发利用提供了理论依据。

The total flavonoids content（ TFC）,total polyphenoliccontent（ TPC）,chemical composition and antioxidant activity of the different solvent extracts of Artemisia sphaerocephala Krasch seeds were investigated. The results showed that the TFC and TPC were correlated with the polarity of solvents used here,the methanol extracts was showed to contain the highest level of TFC（ 33.30 ± 0.11） mg/gand TPC（ 80.1 ± 0.16） mg/g which followed by acetone,ethyl acetate and chloroform extracts.Furthermore,the methanol extracts was the most potent DPPH and ABTS radical-scavenger,the inhibitory concentration 50%（ IC50） were 19.09,26.30 μg/m L,respectively.The ferric reducing activity of the methanol extracts was also higher than the other three extracts. Meanwhile,both quercetin and catechin were validated by HPLC in the methanol extracts with higher content of 5.15,28.24 mg/g,respectively.In short,methanol is suitable for the total polyphenolic and flavonoids extraction from seeds of A.sphaerocephala Krasch,and the extracts might be a valuable antioxidant resource.