响应面法优化米糠多酚的醇提工艺

Optimization of ethanol extraction technique of polyphenols from rice bran by response surface methodology

以大米糠为原料,经脱脂处理后,采用响应面法优化醇提脱脂米糠多酚的提取工艺。在单因素实验的基础上,采用central composite design中心复合响应面分析法,以多酚得率为响应值,对乙醇浓度、料液比和提取温度等工艺参数进行了优化。结果表明,脱脂米糠多酚的最佳提取工艺为乙醇浓度57%,提取温度65℃,料液比1∶25(g/m L),提取时间2 h,提取次数2次。此条件下,脱脂米糠多酚得率最高为(4.75±0.16)mg/g,与回归方程所得预测值4.82 mg/g的相对误差为1.47%。该法所得的提取工艺参数可靠,可为开发利用米糠资源提供依据。

The aim of this paper was to optimize the extraction technique of polyphenols from rice bran by ethanol extraction.Based on the single factor experimental analysis,central composite design of response surface analysis was used to explore the optimal processing conditions to extract polyphenols from rice bran including the concentration of ethanol,extraction temperature and solid to liquid ratio.The results showed that the optimal extraction conditions were the concentration of ethanol57%,extraction temperature of 65 ℃,solid to liquid ratio of 1∶ 25（ g/m L）,extraction duration of 2 h and extraction times of2 times. Under these conditions,the highest yield of polyphenols from defatted rice bran was（ 4.75 ± 0.16） mg/g,the predicted value of the regression equation was 4.82 mg/g,and the relative error was about 1.47%.In conclusion,the optimized extraction process was reliable and could be utilized to develop products based on rice bran.