季铵化β-环糊精接枝壳聚糖涂层用于毛细管电泳分离的研究

Quaternized β-cyclodextrin grafted chitosan as static coating polymer for capillary electrophoresis separation

将β-环糊精接枝到壳聚糖高分子链上,采用醚化试剂使其季铵化,获得季铵化的环糊精接枝壳聚糖。该聚合物可吸附到熔硅毛细管内壁形成静态涂层。在p H 3.0~12.0的范围内,涂层毛细管均能产生强的反向电渗流。利用新涂层对核酸碱基、核苷、氨基酸和蛋白质等不同分子量及不同荷电状态的生物分子进行了电泳分离,获得了较好的分离效果。

The polymer,quaternized β-cyclodextrin grafted chitosan（ QCD-g-CS）,was prepared by reacting chitosan with O-p-toluenesulfonyl-β-cyclodextrin（ Ts-CD） and then etherifying with glycidyltrimethyl ammonium chloride. QCD-g-CS could adsorb onto the inner surface of the silica capillary to form a static coating. Capillaries coated with the polymer were able to generate a strong,reversed electroosmotic flow within a broad range of p H 3. 0 - p H 12. 0. This coating was successfully applied to the CE separation of various biomolecules which possessed different molecular weight and state of charge,namely,nucleic acid bases,nucleosides,amino acids and protein.