摘要
建立液相色谱串联质谱同时检测全血中佐匹克隆、唑吡坦、扎来普隆和褪黑素的方法。采用液液萃取进行提取,提取物以Inersil ODS(2.1×100 mm,3.5μm)色谱柱分离,以10 mmol/L的甲酸铵水溶液(体积分数0.1%甲酸)-乙腈(体积分数0.1%甲酸)为流动相梯度洗脱,流速为0.3 m L/min,液相色谱串联质谱检测。全血中佐匹克隆、唑吡坦和褪黑素的线性范围为0.20~100.0ng/m L,检出限为0.20ng/m L;扎来普隆的线性范围为0.50~100.0ng/m L,检出限0.50ng/m L。
A method for simultaneous determination of zopiclone,zolpidem,zaleplon and melatonin in whole blood by liquid chromatography-tandem mass spectrometry（ LC-MS） was established. Samples were extracted by liquid-liquid extraction. The extracts were separated by an Inersil ODS（ 2. 1 ×100 mm,3. 5 μm） column with 10 mmol/L ammonium formate aqueous solution（ containing 0. 1%formic acid）-acetonitrile（ containing 0. 1% formic acid） as the mobile phase gradient elution,flow rate of 0. 3m L/min,detected by LC-MS. The linear ranges of zopiclone,zolpidem and melatonin in whole blood were all 0. 20 - 100. 0 ng/m L and the detection of limit was 0. 20 ng/m L. The linear range of zaleplon was 0. 50 - 100. 0 ng/m L,and the detection of limit was 0. 50 ng/m L. The method is simple,rapid,accurate and sensitive. It can be used for simultaneous qualitative and quantitative determination of zopiclone,zolpidem,melatonin and zaleplon in blood samples.
出处
《分析试验室》
CAS
CSCD
北大核心
2017年第8期930-933,共4页
Chinese Journal of Analysis Laboratory
基金
公安部科技强警基础工作专项(2015GABJC35)资助
