可逆加成-断裂链转移沉淀法制备孔雀石绿分子印迹材料及应用

Applications of the malachite green imprinted material prepared by RAFT radical precipitation polymerization

采用可逆加成-断裂链转移(RAFT)技术结合分子印迹沉淀聚合法,以孔雀石绿(MG)为模板分子,以二苄基三硫代碳酸酯(DBTTC)为RAFT试剂,制备孔雀石绿分子印迹微球。通过电镜表征,考察溶剂及单体/AIBN/DBTTC的比例对聚合物的形貌的影响,并对其吸附性能进行研究。结果表明,优化条件后制得的聚合物微球粒径均一,分散性好,对目标分子有良好的特异识别性,且吸附量高于传统法。将其做为固相萃取填料,结合高效液相色谱法对小龙虾及养殖水中MG进行分离,富集,检测。方法回收率为77.3%~100.6%,RSD<5.1%,检出限为0.37μg/L。

The malachite green molecularly imprinted microspheres were synthesized by reversible addition-fragmentation chain transfer radical（ RAFT） polymerization and precipitation polymerization,with the dibenzyltrithiocarbonate（ DBTTC） as reversible RAFT reagent. The influence of solvent and ratio of monomer/AIBN/DBTTC on the morphology of the polymer were studied by scanning electron microscope. The adsorption properties of the polymer were studied. The results indicated that the polymer microspheres synthesized under the optimized conditions had uniform particle size,good dispersibility and specific recognition selectivity, of which adsorption was higher than traditional method. The MIP as solid phase extraction（ SPE） materials was used for separation,enrichment of MG in crayfish and aquaculture water with HPLC. The recoveries of the method were in the range of77. 3% to 100. 6%,with RSD less than 5. 1%. The limit of detection was 0. 37 μg/L.