巴马香猪ATP合成酶基因表达发育变化与肉质性状相关性研究

Correlation of expression changes of ATP synthase genes and meat quality in different growth stages of Bama pigs

为了探讨ATP5B、ATP5H基因在巴马香猪和长白猪不同生长阶段背最长肌中的表达丰度与肉质性状之间的关系,揭示ATP合成酶基因对肉质的影响,试验采用荧光定量PCR技术分析了不同时期背最长肌中ATP5B、ATP5H基因mRNA表达量。结果表明:ATP5B、ATP5H基因mRNA表达量具有显著的年龄依赖变化,随着年龄的增加而呈下降趋势,在0日龄时明显高于其他日龄。0日龄时巴马香猪肌肉组织中ATP5B基因mRNA表达量显著高于长白猪(P<0.05),ATP5H基因mRNA表达量显著低于长白猪(P<0.05)。巴马香猪肌肉组织中ATP5B基因mRNA表达量与电导率、剪切力呈显著的负相关性(P<0.05),ATP5H基因mRNA表达量与剪切力呈极显著的负相关性(P<0.01),与肌内脂肪含量呈显著的负相关性(P<0.05)。长白猪肌肉组织中ATP5B、ATP5H基因mRNA表达量与肉色、电导率、剪切力、肌内脂肪含量均无显著的相关性(P>0.05)。

The objective of this study was to explore the conelation of expression abundance of ATPSB and ATPSH genes in longissimus dorsi muscles of different growth stages and meat quality traits in Bama and Landrace pigs, and to reveal the effect of ATP synthase on meat quality. mRNA expression levels of ATPSB and ATPSH genes were analyzed by real - time PCR in longissimus dorsi muscles of different growth stages. The results suggested that the mRNA expression levels of ATI^B and ATI^H were age -dependent,and declined with the increasing age. The levels was significantly higher at 0 day old than that at other days old, mBNA expression level of ATI^B in muscles of Bama pigs was signifi- cantly higher than that in Landrace （ P 〈 0. 05 ） at 0 day old, while the level of ATPSH was significantly lower than that in Landrace（P 〈 0.05 ）. The mRNA expression of ATPSB in Bama pigs was in significantly negative correlation with conductivity or shear force （ P 〈 0.05 ） , and the level of ATPSH and ATPSH in Bama pigs was significantly negative correlation with shear force（ P 〈 0. 01 ） or intramuscular fat （ P 〈 0. 05）. The mR- NA expression of ATPSB in Landrace had no correlation with meat color, conductivity, shear force or intramuscular fat （P 〉 0.05 ）.