肿节风有效成分迷迭香酸对哮喘小鼠Th1/Th2型细胞因子水平的影响

Effects of Zhongjiefeng effective components,rosmarinic acid,on the Th1/Th2 cytokine levels in asthmatic mice

为了探讨肿节风有效成分迷迭香酸对小鼠哮喘模型Th1/Th2细胞因子的调节作用,试验将50只雌性Balb/c小鼠随机分为5组,分别为正常对照组、迷迭香酸单药组、OVA哮喘模型组、迷迭香酸治疗组、地塞米松治疗组,采用ELISA方法对支气管肺泡灌洗液(BALF)中白细胞介素-4(IL-4)、白细胞介素-5(IL-5)、白细胞介素-13(IL-13)和γ干扰素(IFN-γ)水平进行了研究。结果表明:与正常对照组比较,OVA哮喘模型组小鼠BALF中IL-4、IL-5和IL-13水平极显著升高(P<0.01),IFN-γ水平极显著降低(P<0.01),IL-4/IFN-γ比例极显著升高(P<0.01);与OVA哮喘模型组比较,迷迭香酸治疗组和地塞米松治疗组显著抑制了IL-4、IL-5和IL-13水平(P<0.05),显著降低了IL-4/IFN-γ比值(P<0.05)。说明肿节风有效成分迷迭香酸具有抑制哮喘Th2细胞因子和调节免疫平衡的作用。

To explore the effects of Zhongjiofeng effective components, rosmarinic acid, on the regulational function of Th1/Th2 cytokines in asthmatic mice, 50 female Balb/c mice were divided into 5 groups randomly and named control group, siagle rasmarinic acid control group, asthma model group, rosmarinic acid treatment group, dexamethasone treatment group, respectively. Then the levels of IL - 4, IL - 5, IL -13 and IFN - γ in bronchoalveolac lavage fluid （BALF） were detected by the method of ELISA. The results showed that the levels of IL - 4, IL - 5 and IL-13 in BALF were significantly increased in asthma model group compared with the normal group （P 〈0.01 ） , while the level of IFN - T was decreased significantly （ P 〈 0.01 ）, the IL - 4/IFN - γ ratio was significantly increased （P 〈 0.01 ）. Compared with the asthma model group, the IL -4, IL -5 and IL - 13 concentrations and the ratio of IL -4/IFN -γ were significantly decreased in rbsmarinic acid treat- ment group and dexamethasone treatment group （P 〈 0.05 ）, According the results, we concluded that the Zhongjiefeng effective components, resmarinic acid, has effects on the inhibition of Th2 cytokines and the regulational function of immune balance.