圈养成年非洲狮和幼年非洲狮肠道菌群多样性的比较分析

Comparative analysis of the intestinal microbial flora diversity of adult and young African lions

通过PCR-DGGE技术探讨同一养殖环境中的成年非洲狮和幼年非洲狮肠道菌群多样性。提取总DNA,对16S rDNA的V3区进行变形梯度凝胶电泳,对DGGE图谱进行特异性条带回收和克隆测序,通过BLAST与已知条带对比,结果显示:成年非洲狮肠道内含有梭菌属Clostridium)、毛螺旋菌Lachnospiraceae bacterium)、优杆菌属Anaerovorax)、乳球菌属Lactococcus)、消化链球菌属Peptostreptococcus)和Blautia菌属。而幼年非洲狮肠道内菌群较少且均为共有菌群,如:拟杆菌Bacteroidetes bacterium)、瘤胃菌属(rumen bacterium),UPGMA聚类分析得出,成年非洲狮与幼年非洲狮之间菌群结构相似度较低,仅为34%.以上结果表明,非洲狮成长的不同阶段,菌群结构存在明显的差异。这为野生动物不同阶段微生态制剂的研发提供理论基础。

In this study, the intestinal microbial flora diversity of adult and young African lions in the same breeding environment was detected by PCR-DGGE technique. Total bacterial DNA was extracted and 16S rDNA V3 region was amplified,then conducting PCR-DGGE. Subsequently, the specific bands of DGGE were cloned and sequenced. The bacterial species were identified by comparing the sequence through BLAST. The results indicated that the intestinal microbial flora of adult African lions includes Clostridium , Lachnospiraceae bacterium, Anaerovorax , Lactococcus, Peptostreptococcus and Blautia. While tile intestinal microbial flora of young African lions is less- er, most bacteria are common to adult and young lions, such as Bacteroidetes bacterium and rurnen bacterium. The UPGMA clustering analysis of the DGGE fingerprint showed the similarities of the bacteria structures between adult and young African lions were only 34%. These results re- vealed that the intestinal microbial flora has significant difference in different stages of African li- ons. This study lays a foundation for the development of microecological agents in different growth stages of wild animals.