深慢波睡眠剥夺对大鼠睾丸组织氧化应激反应的影响

Impact of deep slow-wave sleep deprivation on oxidative stress response of the testis tissue in rats

目的:研究深慢波睡眠剥夺对大鼠睾丸组织氧化应激反应的影响。方法:36只5周龄健康雄性Wistar大鼠,随机分为深慢波睡眠时相剥夺组(SD1组)、深慢波睡眠时相及时长剥夺组(SD2组)和空白对照组(CC组),每组12只,利用小平台水环境建立深慢波睡眠剥夺模型。SD1组每间隔24 min干扰1次,SD2组每间隔24min剥夺睡眠8 min;夜间都进行12 h的完全睡眠剥夺。CC组模拟正常的12 h光照、12 h黑暗时间。28 d后,大鼠股动脉放血,留取睾丸组织进行称重,测定睾丸组织中蛋白含量、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物岐化酶(SOD)水平,显微镜下观察睾丸病理结构的改变。结果:SD1、SD2组大鼠的终末体质量[(248.1±25.1)g、(232.9±10.1)g]均下降,与CC组[(268.5±1.6)g]相比差异有统计学意义(P均<0.05);与CC组相比,SD2组睾丸相对质量明显升高[(54.9±3.5)×10^(-2)vs(50.0±1.3)×10^(-2),P<0.05]。与CC组相比,SD2组睾丸组织蛋白含量、MDA含量、SOD活性和GSH-Px均有显著差异[蛋白含量:(4.5±0.9)g pro/L vs 6.3±1.4)g pro/L;MDA含量:(1.3±0.3)nmol/mg pro vs(1.1±0.1)nmol/mg pro;SOD活性:(135.2±26.9)U/mg pro vs(104.3±33.1)U/mg pro;GSH-Px活性:(21.7±4.3)U/mg pro vs(15.6±4.0)U/mg pro,P均<0.05],而与SD1组比较差异无统计学意义。SD1组睾丸病理学改变不明显,但SD2组睾丸生精小管管腔变小,周围间质部分增加,间质充血水肿明显。结论:长期深慢波睡眠剥夺对大鼠睾丸组织结构产生损伤并引起氧化应激反应。

Objective ： To investigate the influence of deep slow-wave sleep deprivation on the oxidative stress of testicular tissue in rats. Methods： Thirty-six 5-week-old male Wistar rats were equally randomized into deep slow-wave sleep deprivation group （SD1）, deep slow-wave and duration sleep deprivation group （SD2）, and a cage control group （CC）. The rat model of deep slow-wave sleep deprivation was established using the flowerpot technique. The rats in the SD1 group were interfered every 24 minutes and deprived of 12 hours of sleep at night, those in the SD2 group deprived of 8 minutes of sleep at an interval of 24 minutes and 12 hours of sleep at night, and those in the CC group exposed to 12 hours of daylight and 12 hours of darkness. After 28 days, all the rats were executed for measurement of the testis volume and protein content, determination of the methane dicarboxylic aldehyde （MDA） level and activities of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px）, and observation of the pathological changes in the testicular tissue under the microscope. Results ： Compared with the CC group, the rats in the SDI and SD2 groups showed significantly reduced body weight （ [268.5 ± 1.6] vs [248.1 ± 25.1] and [232.9± 10.1] g, P 〈0.05） and increased relative testis mass （ [ 50.0 ± 1.3 ] vs [ 57.9 ± 6.1 ] and [ 54.9± 3.51]×10^-2, p 〈 0.05）. Statistically significant differences were found between theCCandSD2 groups in the contents of protein （[6.3 ± 1.4] vs [4.5 ± 0.9] gpro/L, P 〈0.05） andMDA （[1.1 ± 0.1] vs [ 1.3 ± 0.3 ] nmol/mg pro, P 〈 0.05） and the activities of SOD （ [ 104.3 ± 33.1 ] vs [ 135.2 ± 26.9] U/rag pro, P 〈 0.05） andGSH-Px （[15.6 ± 4.0] vs [21.7 ± 4.3] U/mgpro, P 〈0.05）, but not between theCCandSD1 groups （P 〉0.05）. The lumens in the testis were narrowed, with obvious hyperplasia, hyperemia and edema in the peripheral interstitial tissue, but no significant pathologic changes were observed in the testis tissue of the SD1 group. Conclusion ： Long-term deprivation of deep slow-wave sleep impairs the structure of the testis tissue and induces oxidative stress response in rats.