转录因子Twist、Snail在宫颈鳞癌中的表达及其与血管生成的关系

Relationship of Expression of Twist and Snail with Angiogenesis in Cervical Squamous cancer

目的探讨Twist、Snail蛋白在宫颈鳞癌组织中的表达情况及其与微血管密度的关系。方法采用免疫组织化学方法对60例宫颈鳞癌组织Twist及Snail蛋白的表达进行检测,分析其与临床病理特征的关系。CD34抗体标记癌组织内血管内皮细胞数量,计算微血管密度(MVD)。结果宫颈鳞癌组织中Twist及Snail的阳性表达均与患者的组织学分级及临床分期无关(P>0.05),与淋巴结转移有关(P<0.05)。Twist及Snail阳性表达组MVD显著高于Twist、Snail阴性组。在宫颈鳞癌组织中Twist及Snail蛋白的表达之间存在显著正相关(r=0.529,P<0.05)。结论宫颈鳞癌组织中存在Twist及Snail蛋白的高表达,两者的联合表达可能增加肿瘤细胞的侵袭力,并且可以通过微血管生成在宫颈鳞癌的浸润、转移过程中发挥促进作用。

Objective To study the expression of Twist and Snail in cervical squamous cancer and their relationship with angiogenesis.Methods Expression of Twist and Snail was detected in 60 cases of cervical squamous cancer tissue by immunohistochemistry.CD34 marking vascular endothelial cell was used to measure the microvascular density（MVD）.Results The positive Twist expression was 88.3% in cervical squamous cancer,and the positive Snail was 78.3%. The positive group of Twist and Snail protein had higher MVD than that in negative group（P〈0.05）. There was a significant correlation between the expressions of Twist and Snail in cervical squamous cancer（P〈0.05）.Conclusion The overexpression of Twist and Snail protein were found in cervical squamous cancer.Their co-expression could contribute to the invasion and metastasis in human cervical squamous cancer.They were valuable molecular markers to be used for monitoring the disease.