生物膜层干涉技术在生物分子分析和检测中应用的研究进展

Research Progress in Application of Biolayer Interferometry in Analysis and Detection of Biomolecules

生物膜层干涉技术是基于光干涉信号实现对生物分子动力学分析或快速检测的非标记分析检测技术。因具有实时提供分析物信息、分析速度快和样品耗量少等优点,该技术已被应用于蛋白质、核酸、脂质、糖类及其他生物分子之间相互作用的分析;所需样品量少、特异性强等优点也为该技术应用于快速检测奠定了基础。该文介绍了生物膜层干涉技术的基本原理,综述了其在动力学分析和快速检测领域的相关应用研究,并对其发展趋势和应用前景进行了展望。

Biolayer interferometry is an optical system that monitors interactions between biological materials and detects biological materials rapidly for lable-free. Due to its advantages of real-time, rapidness and low sample consumption, biolayer interferometry has been gradually used for detecting interactions between proteins, peptides, nucleic acids, small molecules, and/or lipids. Further- more, biolayer interferometry could also provide a solid foundation in rapid detection field because of its low sample consumption and strong specificity. In this article, the principle of biolayer interfer- ometry is discussed, and two major application areas including kinetic analysis and rapid detection are investigated and reviewed. The development trend and future application of biolayer interferometry for measuring biological materials are also prospected.