环境压力条件与超高压对致病性副溶血性弧菌耐药性的影响（英文）

Effect of environmental stresses and high hydrostatic pressure on the Antibiotic susceptibility of pathogenic Vibrio parahaemolyticus

分析不同环境压力条件(温度、pH、渗透压和超高压)对12株致病性副溶血性弧菌耐药性的影响。利用微量肉汤稀释法测定菌株在不同温度(37℃和30℃)、渗透压(1%和6%NaCl)、pH(6.0和9.0)及超高压(180、250和300 MPa)条件影响下,其对所测抗生素的最小抑菌浓度(MIC)。结果表明,在1%和6%NaCl质量分数、pH 9.0条件下,副溶血性弧菌的耐药性增强,而在30℃、pH 6和超高压条件影响下,其耐药性减弱。此外,菌株在所测环境压力条件影响下,其对环丙沙星和头孢类抗生素的耐药性基本保持不变。环境压力条件的改变可能会增强致病性副溶血性弧菌的耐药性。

The aim of the present study was to determine the effect of environmental stresses（ temperature,pH,osmotic pressure,high hydrostatic pressure（ HHP）） on the antibiotic susceptibility of 12 different pathogenic Vibrio parahaemolyticus isolates. Isolates were subjected to growth at（ 30 ℃ and 37 ℃）,an osmotic pressure of（ 1% and 6% NaCl）,a media at pH（ 6. 0 and 9. 0） and three different HHP treatment（ 180,250,300 MPa）. The minimal inhibitory concentrations（ MICs） of tested antibiotics used against unstressed（ control）,stressed or post-stressed isolates were determined using the broth microdilution method. The study found that incubation under increased salt（ 6%）,reduced salt（ 1%） and increased pH（ 9. 0） conditions were commonly associated with increased antibiotic resistance. Incubation at 30 ℃ temperature,reduced pH（ 6. 0） and HHP treatment were commonly associated with decreased antibiotic resistance. Besides,both ciprofloxacin and cefotaxime kept a constant MIC during almost all stress challenges. Thus our data demonstrate that exposure of V. parahaemolyticus to some stress conditions may contribute on the rapid development of antibiotic resistance in this food borne pathogen.