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新生儿肺泡表面活性物质蛋白A基因多态性与肺炎易感性、炎症反应程度的相关性 被引量:12

Correlation of neonatal pulmonary surfactant protein A gene polymorphism with pneumonia susceptibility and inflammatory response
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摘要 目的:研究新生儿肺泡表面活性物质蛋白A基因多态性与肺炎易感性、炎症反应程度的相关性。方法:选择在本院出生且诊断为肺炎的新生儿作为肺炎组,非感染新生儿作为对照组。测定SP-A基因rs1059054和rs1136454位点的多态性、血清中炎症细胞因子的含量、外周血炎症转录因子的表达量。结果:肺炎组新生儿rs1059054位点CC基因型的构成比显著高于对照组,CT、TT基因型的构成比显著低于对照组;rs1136454位点AA基因型的构成比显著低于对照组,AG、GG基因型的构成比显著高于对照组。SP-A基因rs1059054位点CC基因型肺炎患儿血清中PCT、sTREM1、TNF-α、IL-6的含量以及外周血中RORγt的mRNA表达量显著高于CT基因型、TT基因型肺炎患儿,外周血中SOCS1、Foxp3的mRNA表达量显著低于CT基因型、TT基因型肺炎患儿;SP-A基因rs1136454位点AA基因型肺炎患儿血清中PCT、sTREM1、TNF-α、IL-6的含量以及外周血中RORγt的mRNA表达量显著低于AG基因型、GG基因型肺炎患儿,外周血中SOCS1、Foxp3的mRNA表达量显著高于AG基因型、GG基因型肺炎患儿。结论:新生儿SP-A基因rs1059054位点CC基因型会增加肺炎易感性并加重炎症反应,rs1136454位点AA基因型会降低肺炎易感性并减轻炎症反应。 Objective:To study the correlation of neonatal pulmonary surfactant protein A gene polymorphism with pneumonia susceptibility and inflammatory response.Methods:Neonates who were born and diagnosed with pneumonia in Zigong Maternity and Child Healthcare Hospital between September 2015 and February 2017 were selected as pneumonia group,and neonates without infection were selected as control group.SP-A gene rs1059054 and rs1136454loci polymorphism,the contents of inflammatory cytokines in serum as well as the expression of inflammatory transcription factors in peripheral blood were determined.Results:The constituent ratio of rs1059054 loci CC genotype of pneumonia group was significantly higher than that of control group while the constituent ratio of CT and TT genotypes were significantly lower than those of control group;the constituent ratio of rs1136454 loci AA genotype was significantly lower than that of control group while the constituent ratio of AG and GG genotypes were significantly higher than those of control group.PCT,sTREM1,TNF-αand IL-6levels in serum as well as RORγt mRNA expression in peripheral blood of pneumonia children with SP-A gene rs1059054 loci CC genotype were significantly higher than those of pneumonia children with CT genotype and TT genotype while SOCS1 and Foxp3mRNA expression in peripheral blood were significantly lower than those of pneumonia children with CT genotype and TT genotype;PCT,sTREM1,TNF-αand IL-6levels in serum as well as RORγt mRNA expression in peripheral blood of pneumonia children with SP-A gene rs1136454 loci AA genotype were significantly lower than those of pneumonia children with AG genotype and GG genotype while SOCS1 and Foxp3 mRNA expression in peripheral blood were significantly lower than those of pneumonia children with AG genotype and GG genotype.Conclusion:Neonatal SP-A gene rs1059054 loci CC genotype can increase the pneumonia susceptibility and aggravate inflammatory response,and rs1136454 loci AA genotype can decrease pneumonia susceptibility and relieve inflammatory response.
作者 何艺 HE Yi(Neonatology Department, Zigong Maternity and Child Healthcare Hospital in Sichuan Province, Zigong 643000, Chin)
出处 《海南医学院学报》 CAS 2017年第12期1668-1671,共4页 Journal of Hainan Medical University
基金 四川省教育厅资助项目(14zb059)~~
关键词 新生儿肺炎 肺泡表面活性物质蛋白A 基因多态性 炎症反应 Neonatal pneumonia Pulmonary surfactant protein A Gene polymorphism Inflammatory response
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