miRNA-210在人脑胶质瘤中表达的临床意义及其生物学效应的探究

The clinical significance of miRNA-210 expression in human glioma and its biological effects

目的:探究微小核糖核酸-210(miRNA-210)在脑胶质瘤患者组织中表达特征和其临床意义,以及其对胶质瘤细胞增殖侵袭能力的影响。方法:收集我院神经外科收治的42例脑胶质瘤及20例良性脑膜瘤患者病变组织,实时荧光定量PCR(Quantitative Real-time PCR,qPCR)检测患者组织中miRNA-210表达水平并采用Kaplan-Meier生存分析比较其与患者预后的关系。利用体外转染小干扰RNA(siRNA)技术干扰人脑胶质瘤细胞U251的miRNA-210表达,MTT法检测细胞增殖能力变化,Transwell侵袭实验检测细胞侵袭能力变化。结果:miRNA-210在29例高级别脑胶质瘤中的表达高于13例低级别脑胶质瘤及20例良性脑膜瘤的表达(P<0.05);Kaplan-Meier生存曲线示高表达miRNA-210的患者预后较低表达的患者不良(P<0.05);成功转染siRNA至U251细胞中,干扰组U251细胞(U251simiR-210)miR-210表达相比对照细胞(U251control)显著下降,U251simiR-210细胞在72h时MTT溶液相对吸光度显著低于U251control细胞,U251simiR-210细胞穿透基质膜细胞数也显著少于U251control细胞。结论:miRNA-210的表达与脑胶质瘤恶性程度及患者预后密切相关,其可促进胶质瘤细胞的增殖侵袭能力,是脑胶质瘤中重要的促癌基因。

Objective：To investigate the expression and clinical significance of microRNA-210（miRNA-210）in glioma tissues and its effect on the proliferation and invasion of glioma cells.Methods：A total of 42 cases of brain glioma and 20 cases of benign meningioma were collected in the department of neurosurgery in our hospital.Quantitative Real-time PCR（qPCR）was used to detect the expression of miRNA-210 in tissue of patients,and Kaplan-Meier survival analyzed the relationship between the expression and the patients prognosis.Small interference RNA（siRNA）was used to interfere the expression of miRNA-210 in U251human glioma cell line in vitro.MTT assay was used to detect the ability of cell proliferation.Transwell invasion assay was used to detect the ability of cell invasion.Results：：The expression of miRNA-210 in 29cases of high grade gliomas was higher than that of 13 cases of low grade gliomas and 20 cases of benign meningioma（P〈0.05）.Kaplan-Meier survival showed the prognosis glioma patients with high expression of miRNA-210 was poorer than patients with low expression（P〈0.05）.SiRNA was transfected to U251 cells successfully,and the expression of miR-210 in U251simiR-210 cell was decreased significantly compared to U251 control.The Results：of MTT showed the solution relative absorbance of U251simiR-210 cells was significantly lower than that of U251 control cell in 72h（P〈0.05）.The Results：of MTT assay showed the solution relative absorbance of U251simiR-210 cells was significantly lower than that of U251 control cell in 72 h.The Results：of transwell invasion assay showed the cell number of penetrating membrane in U251simiR-210 cells was significantly less than that of U251 in control cells.Conclusions：The expression of miRNA-210 is closely related to the malignant degree and prognosis of glioma patients,which can promote the proliferation and invasion of glioma cells.