淫羊藿苷对哮喘大鼠肾上腺皮质功能的影响

Influence of Icariin on Adrenal Cortex Function in Rats with Asthma

目的观察淫羊藿苷对哮喘大鼠的治疗作用及其对哮喘大鼠肾上腺皮质功能的影响。方法健康雄性挪威棕色大鼠40只,被随机分为4组:正常对照组,哮喘模型组,淫羊藿苷组,地塞米松对照组,每组10只。采用卵蛋白致敏及雾化吸入激发方法制备哮喘大鼠模型,观察肺泡灌洗液中嗜酸性粒细胞计数的变化,采用苏木精-伊红染色观察各组大鼠肺组织病理变化;采用酶联免疫吸附试验检测血清中皮质酮水平;采用肾上腺质量指数评价各组大鼠肾上腺皮质情况,同时分离肾上腺细胞,流式细胞仪检测细胞凋亡率。结果与正常对照组比较,哮喘模型组大鼠肺组织呈现明显的炎性反应,肺泡灌洗液中嗜酸性粒细胞计数明显升高(P<0.05),血清中皮质酮水平、肾上腺质量指数均降低(P<0.05),肾上腺细胞的凋亡率升高(P<0.05)。淫羊藿苷和地塞米松均能够明显减轻模型大鼠肺组织的炎性反应,降低肺泡灌洗液中嗜酸性粒细胞计数(P<0.05);与哮喘模型组大鼠比较,淫羊藿苷能升高哮喘大鼠血清中皮质酮水平,提高肾上腺质量指数和降低肾上腺细胞的凋亡率(P<0.05)。结论淫羊藿苷防治哮喘的机制可能与提高哮喘大鼠的肾上腺皮质功能有关。

Objective To investigate the therapeutic effect of icariin and its influence on adrenal cortex function in rats with asthma.Methods A total of 40 healthy male BN rats were randomly divided into normal group, model group, icariin group, and dexamethasone control group, with 10 rats in each group.Ovalbumin sensitization and challenge with aerosol inhalation were performed to establish a rat model of asthma.The change in eosinophil count in bronchoalveolar lavage fluid was observed.Hematoxylin and eosin staining was used to observe lung pathological changes, and enzyme-linked immunosorbent assay was used to measure the serum level of corticosterone.The adrenal mass index was used to evaluate the adrenal cortex in each group, and adrenal cells were isolated and flow cytometry was used to measure cell apoptosis rate.Results Compared with the normal group, the model group had marked inflammatory response in lung tissue, a significant increase in eosinophil count in bronchoalveolar lavage fluid （P〈0.05）, significant reductions in the serum level of corticosterone and adrenal mass index （P〈0.05）, and a significant increase in the apoptosis rate of adrenal cells （P〈0.05）.Both icariin and dexamethasone significantly alleviated inflammatory response in lung tissue and reduced eosinophil count in bronchoalveolar lavage fluid （P〈0.05）.Compared with the model group, the icariin group had significant increases in the serum level of corticosterone and adrenal mass index and a significant reduction in the apoptosis rate of adrenal cells （P〈0.05）.Conclusion The mechanism of action of icariin in the prevention and treatment of asthma may be related to the improvement in adrenal cortex function in rats with asthma.