P16、P15基因在胃癌及转移淋巴结中的表达

Expression of P16 and P15 in human gastric carcinoma and metastatic lymph node

目的 :观测抑癌基因P16、P15在原发性胃癌及淋巴结中的表达 ,以探讨两者在胃癌的发生转移及预后中的作用。方法 :应用免疫组织化学检测 92例原发性胃癌及 138枚转移淋巴结中P16、P15蛋白的表达。结果 :P16、P15阳性表达率在原发癌中分别为 17 4 %和 2 8 3% ,在转移淋巴结中分别为 15 9%和 2 8 3% ,原发癌伴淋巴结转移组P16、P15阳性表达率较无淋巴结转移组显著低 (P <0 0 5 ) ,P16、P15表达在原发性胃癌中呈正相关性 (P<0 0 5 ,列联系数r=0 2 74 ) ,在淋巴结中呈正相关 (P <0 0 5 ,列联系数r =0 32 4 )。结论 :P16、P15基因蛋白对胃癌的转移可能有抑制作用 ,两者联合检测对胃癌淋巴结转移预测及预后评估具有一定帮助。

Purpose:To investigate the expression of tumor suppressor genes P16 and P15 in human primary gastric carcinoma(PGC) and metastatic lymph node(MLN) in order to explore their roles in carcinoma metastasis and the prognosis of PGC.Methods:The expresion of P16 and P15 was detected by SP immunohistochemical method in 92 cases of PGC and 138 MLN. All samples were obtained fresh, fixed with 10% buffered formalin immediately, embedded with paraffin and then sectioned serially. Results:The positive expression rates of P16 and P15 were 17.45% and 28.3% in PGC, and 15.95% and 28.3% in MLN respectively. The positive expression rates of P16 and P15 in PGC with lymph node metastasis was lower than that without lymph node metastasis ( P <0.05) . There is a significant positive correlation between the expression of P16 and that of P15 in PGC( P <0.05, r=0.274) , and in MLN( P <0.05, r=0.324).Conclusions:The P16?P15 protein may play an important role in suppression of PGC metastasis. The combined detection of P16 and P15 protein may be helpful in evaluation of lymph node metastasis and prognosis of PGC.