桑芪首乌片对自发性高血压大鼠的降压作用和肾素-血管紧张素系统的影响

Effects of Sangqi Shouwu Decoction on Blood Pressure and Renin Angiotensin System in Spontaneously Hypertensive Rats

目的观察桑芪首乌片对高血压大鼠的降压作用并探讨其机制。方法 SHR大鼠60只,随机分为低、中、高3个剂量实验组,卡托普利组、复方丹参组和空白对照组,每组10只。同时10只SD大鼠为正常对照组。采用无创尾动脉血压测量系统监测受试后24 h、7 d、14 d、21 d、35 d和给药结束1周测量血压,同时测定给药后7 d、14 d、35 d及停药后7 d血浆BK、AngⅠ、AngⅡ水平变化情况。结果各给药组收缩压与正常对照组与空白对照组差异均有统计学意义(P<0.05),但各组之间比较差异无统计学差异(P>0.05)。给药后,各观察组血浆AngⅠ、AngⅡ水平明显低于空白对照组(P<0.05)。给药后,各观察组BK水平明显高于空白对照组(P<0.05)。结论本研究表明,桑芪首乌片可明显降低SHR大鼠血浆AngⅠ、AngⅡ水平,上调SHR大鼠血浆BK水平,提示桑芪首乌片作用有可能通过抑制肾素转化AngⅠ,拮抗机体ACE的活性,减少AngⅡ的生成而发挥作用,对RAS的作用具有多途径、多环节、多靶点的综合影响。

Objective： To study the antihypertensive effect of Sangqi Shouwu Decoction on hypertensive rats and its mechanism. Methods： 60 SHR rats were randomly divided into low, medium and high three dose groups, cap- topril group and Fufang Dashen group and blank control group, 10 in each group. At the same time, 10 SD rats were in the normal control group. Noninvasive caudal artery BPMS was used to monitor blood pressure, as well as the change of BK,Ang I and Ang II ,in 24 h and on 7 d,14 d,21 d and 35 d after treatment. Results： After treatment,the systolic blood pressure of each adminstration groups was statistically significant compared with the normal contral group and the blank control group （P〈 0.05）,but there was no statistically significant difference between the remaining groups（P〉 0.05）. After administration, the levels of Ang I and Ang 11 in other groups were obviously lower than those in the blank control group （P〈 0.05）,and the difference was statistically significant. After administration, the levels of BK in other groups were obviously higher than that of the SHR control group （P〈 0.05）,and the difference was statistically significant. Conclusion： This study shows that Sangqi Shouwu De- coction can significantly reduce the levels of Ang I and Ang II in plasma of SHR rats and up-regulate the plas- ma BK level in SHR rats, suggesting that the effect of Sangqi Shouwu Decoction may inhibit the expression of Ang I ,ACE activity,reduce the formation of Ang II and play a role in the RAS has been a multi-channel,multi- link, multi-target integrated effects.