青柏溃结汤对溃疡性结肠炎大鼠疗效的研究

Study on Effects of Qingbai Kuijie Decoction on Ulcerative Colitis Rats

目的观察青柏溃结汤对乙酸诱导溃疡性结肠炎大鼠模型结肠黏膜愈合及血清细胞因子的影响并探讨其机制。方法将77只雄性SD大鼠随机分为空白组、模型组、青柏溃结汤高剂量组、青柏溃结汤中剂量组、青柏溃结汤低剂量组、柳氮磺吡啶组、康复新液组,各11只,除空白组外其余各组采用乙酸造模。造模后第3开始灌肠给药,连续7 d,随后观察各组结肠组织并评分。用ELISA检测血清肿瘤坏死因子-α(TNF-α)、白介素-10(IL-10)含量。结果与模型组比较,各治疗组血清TNF-α含量下降、IL-10含量升高。结论青柏溃结汤能明显减轻溃疡性结肠炎大鼠结肠组织的损伤程度,这可能与其下调TNF-α、上调IL-10的水平有关。

Objective： To observe the effect of Qingbai Kuijie Decoction on the healing of colonic mucosa and serum cytokines in rats with ulcerative colitis induced by acetic acid. Methods： 77 male SD rats were randomly divided into the control group,the model group,high dose group of Qingbai Kuijie Decoction,middle dose group of Qingbai Kuijie Decoction,low dose group of Qingbai Kuijie Decoction,sulfasalazine group and Kangfuxin Decoc- tion group, 11 cases in each. Except the control group, the remaining groups were modeled with acetic acid. Three days after modelling,the rats were given enema for 7 days. Then the colon tissues of each group were observed and scored. Serum levels of TNF-α and IL-10were detected by ELISA. Results： Compared with the model group, the serum levels of TNF-α decreased and the content of IL-10 increased in the other groups. Conclusion.. Qingbai Kuijie Decoction can significantly alleviate the damage degree of colon tissue in rats with ulcerative coli- tis,which may be related to the down-regulation of TNF-α and up-regulation of IL-10.