陆地棉光合系统Ⅱ GhPsbS基因的克隆及其表达分析

Cloning and Expression Analysis of Photosystem Ⅱ GhPsbS Gene in Gossypium hirsupum

以拟南芥光合系统Ⅱ PsbS蛋白序列为探针,采用电子克隆与同源克隆结合的方法得到陆地棉光合系统Ⅱ PsbS基因的cDNA序列(GenBank No.ANS53414),进行序列分析,并对其在不同处理下的表达水平进行研究。结果表明:该基因开放阅读框全长837 bp,编码278个氨基酸,蛋白分子量大小为22 kD,为疏水性蛋白,含有两个典型的捕光叶绿素a/b结合蛋白功能域,属于典型的叶绿素a/b结合蛋白家族中的成员。qRT-PCR分析表明,强光、弱光、干旱、高盐、低温胁迫处理后,该基因的表达量整体均呈下降趋势,推测该基因不仅能响应不同光照处理,还能够响应干旱、高盐和低温胁迫,尤其受弱光、高盐和干旱胁迫的诱导表达较为明显。该研究为进一步了解GhPsbS基因对陆地棉高光效育种及抗逆机制的研究提供帮助。

The cotton （Gossypium hirsupum） PsbS cDNA （GenBank No. ANS53414） was cloned by means of in silicon cloning and homology cloning with Arabidopsis thaliana PsbS protein sequence as a probe. Then the sequence was analyzed, and the expression level in different treatments of cotton was studied. The results showed that the open reading frame of the gene was 837 bp, encoded a 22 kD protein of 278 amino acids. It was a hydrophobic protein and contained two typical chlorophyll a/b binding protein domains, which belonging to the typical chlorophyll a/b binding protein family. The results of qRT-PCR analysis showed that the expression level of this gene was decreased after stress treatments with high light, low light, drought, high salt and low temperature stress, so the gene could not only respond to different light treatments, but also respond to drought, high salt and low temperature stress, especially under low light, high salt and drought stress induction of expression could be more obvious. This study will provide a basis for further study on GhPsbS gene in the breeding of high light efficiency and the mechanism of stress resistance in upland cotton.