胡桃楸成花相关LFY同源基因的克隆和序列分析

Cloning and Analysis of Flowering Homologous Gene LFY from Juglans mandshurica Maxim

通过对胡桃楸成花相关LFY基因进行研究,对缩短胡桃楸童期,促进其提早开花结实有着极为重要的意义。采取PCR扩增技术,在NCBI数据库中进行搜索,查找到与目的基因有关联的同源序列;并根据这些有关联的序列来进行引物设计,扩增目的基因全长。克隆LFY基因cDNA序列全长为1 297 bp,其中CDS序列长1 158 bp,编码385个氨基酸。NCBI数据库Blast比对表明胡桃楸LFY同源基因与其他物种的LFY同源基因同源性均在80%以上。将胡桃楸LFY同源基因命名为JmLFY(GenBank登录号:KX364241)。通过BioEdit软件和ProtParam ExPASy在线软件分析胡桃楸JmLFY蛋白质分子式为C_(1878)H_(2962)N_(566)O_(578)S_(13),分子量为43 134.4 Da;理论等电点pI值为6.78;不稳定指数为39.92,属于不稳定蛋白质。该研究为进一步探索LFY基因对胡桃楸成花的影响提供依据。

It has a very important significance to shorten the junior period and promote blossoming by studing the flowering gene LFY from Juglans mandshurica Maxim. Using the PCR amplification technique and NCBI database information searching method, we found the homologous sequences of target gene fragment. The sequences were used to design primers and amplify full-length gene. The full-length of c DNA sequence cloned by the LFY gene was 1 297 bp, the sequence length of CDS was 1 158 bp encoding 385 amino acids. The result of Blast comparison in NCBI datebase indicated that the homology of LFY homologous gene from Juglans mandshurica Maxim and other species were more than 80%. The Juglans mandshurica Maxim homologous gene were named as Jm LFY（Gen Bank accession number： KX364241）. Through the Bio Edit and Prot Param Ex PASy software, we knew the molecular formula of protein Jm LFY was C1878H2962N566O578S13, the molecular weight was43 134.4 Da, the theory of isoelectric point was 6.78, the instability index was 39.92. It was classified as unstable protein. This research could provide the basis for further exploring the effects of LFY gene on Juglans mandshurica Maxim flowering.