摘要
为获得刺五加FPS的DNA和启动子序列,以及其功能元件信息和基因结构,根据已克隆的FPS cDNA序列设计引物,使用TAIL-PCR技术克隆FPS的基因组DNA及启动子序列,再通过各生物软件分析基因结构与启动子功能元件。得到刺五加FPS基因全长为7 952 bp,含有12个外显子和11个内含子,启动子序列含有25个类型功能元件,包括:62个TATA-box、24个CAAT-box,以及其他类型的重要功能元件34个。本研究首次克隆得到刺五加FPS的DNA序列和启动子全长,获取了内外显子的分布情况以及启动子功能元件,为后续进一步研究FPS在刺五加中的表达调控奠定了基础。
To clone FPS gene DNA sequence and promoter sequence from Eleutherococcus senticosus, get the information of functional components and biology software. Be based on the cDNA of FPS to design primers, and using TAIL-PCR technology to clone the sequence of DNA and promoter. Then analyzed them with some biology software to find genetic structures and functional components. The length of FPS is 7 952 bp, it includes 12 exons and 11 introns, and its promoter contains 25 types of functional components, such as 62 TATA-box, 24 CAAT-box and 34 other types of ones. In this research FPS genomic DNA and promoter sequence was cloned from E.senticosus in the first time. And get structures and functional components of FPS, it establishes foundation for studying FPS expression of regulation mechanism.
作者
宋菊
林丽梅
尤鹏升
国红玉
龙月红
邢朝斌
Song Ju Lin Limei You Pengsheng Guo Hongyu Long Yuehong Xing Zhaobin(College of Life Science, North China University of Science and Technology, Tangshan, 06300)
出处
《分子植物育种》
CAS
CSCD
北大核心
2017年第7期2584-2589,共6页
Molecular Plant Breeding
基金
国家自然科学基金项目(31570683)
河北省教育厅资助科研项目(QN2014102)
华北理工大学研究生创新项目(2017S12)
华北理工大学培育基金项目(SP201508)共同资助
关键词
刺五加
法尼基焦磷酸合酶
启动子
生物信息学分析
Eleutherococcus senticosus
Farnesyl diphosphate synthase
Promoter
Bioinformatic analysis
