摘要
[目的]探讨乙肝病毒感染患者中乙肝两对半模式和乙肝病毒DNA定量相关性。[方法]以在三亚市人民医院就诊的乙型肝炎患者500例为研究对象,分别采用酶联免疫吸附法及荧光定量PCR法测定其乙肝两对半及乙肝病毒DNA(HBV-DNA)定量,观察两者之间的相关性。[结果]共纳入乙型肝炎患者500例,其中大三阳患者HBV-DNA定量对数值显著高于其他组(P<0.05;e抗原定量与HBV-DNA对数定量相关分析显示:e抗原阳性患者中,e抗原定量值与HBV-DNA对数定量呈高度线性相关(r=0.81);e抗原阴性患者e抗原定量值与HBVDNA对数定量呈中度线性相关(r=0.52)。[结论]乙型肝炎患者中乙肝两对半模式与HBV-DNA定量值存在明显相关性,两者联合检测可准确反映乙型肝炎患者体内乙肝病毒复制情况,值得临床借鉴参考。
[Objective]To investigate the correlation of"two and a half pairs of hepatitis B"models and HBV DNA quantitative in HBV infected patients.[Methods]500 patients with hepatitis B in our hospital,as the research object,and the determination of the two semi hepatitis B and hepatitis B virus DNA(HBVDNA)were assayed by enzyme linked immunosorbent assay and fluorescence quantitative PCR quantitative.The correlation was observed between the two modes.[Results]The study included 500 patients with hepatitis B,among them whose surface antigen,e antigen and core antibody positive,HBV-DNA numerical quantification were significantly higher than that in other groups(P〈0.05);e antigen quantitative correlation with HBV-DNA log quantitative analysis showed that in e antigen positive patients,the quantitative value of e antigen was high linear correlated with the logarithm of HBV-DNA(r=0.81);In e antigen negative patients,the quantitative value of e antigen was moderately linear correlation with the logarithm of HBV-DNA(r=0.52).[Conclusion]There is a significant correlation between hepatitis B two-pair model and HBV-DNA quantification in patients with hepatitis B,and both joint detection can accurately reflect the hepatitis B virus replication in vivo,it is worthy for clinical reference.
作者
方菊
李欢
綦鹏
陈开俏
FANG Ju LI Huan QI Peng CHEN Kaiqiao(Department of Laboratory, Maternal and Child Health Hospital of Sanya ,Hainan 572000 China Department of Laboratory, the People's Hospital of Sanya City, Hainan 572000 China Department of Internal Medicine,the People's Hospital of Sanya City, Hainan 572000 China)
出处
《中国中西医结合消化杂志》
CAS
2017年第7期529-531,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
关键词
乙型肝炎
两对半
乙肝病毒
DNA定量
Hepatitis B
Two pairs of half
Hepatitis B virus
DNA quantitative