双氢睾酮和氟他胺干预前列腺癌细胞LNCaP线粒体内还原型谷胱甘肽的变化

The change of GSH on the mitochondrion in prostate cancer cells LNCaP by DHT and FLU

目的 观察双氢睾酮（DHT）和氟他胺（FLU）作用于雄激素依赖性（LNCaP）前列腺癌细胞LNCaP线粒体自由基的变化,探讨DHT和FLU引起LNCaP细胞线粒体内还原型谷胱甘肽（GSH）变化的作用机制.方法 获得LNCaP细胞株,进行细胞培养,获得稳定生长及传代的细胞株.不同浓度的雄激素受体激动剂DHT及雄激素受体拮抗剂FLU作用于培养的LNCaP细胞株;不同时间点提取干预措施下的LNCaP细胞线粒体;通过检测并比较不同时间点、不同浓度药物作用下LNCaP细胞株线粒体自由基的变化和差异.结果 DHT作用于LNCaP细胞后,线粒体GSH的含量显著下降（P〈0.05）,各个指标在不同的时间点上下降的程度有所差异.FLU干预LNCaP后,中剂量组干预14 d与对照组比其GSH含量增加（P〈0.05）.其余各组GSH的含量差异无统计学意义（P〉0.05）.结论 DHT可明显降低LNCaP线粒体内GSH含量,说明可促进细胞的增殖活力;FLU不能清除LNCaP细胞线粒体GSH,导致自由基蓄积,可有效抑制LNCaP细胞的增殖.

Objectives To investigate the change of double hydrogen testosterone（DHT）and flutamide（FLU）on the mitochondrion in prostate cancer cells LNCaP.Methods Drogen-dependent（LNCaP）prostate cancer cell lines and cell lines with stable growth and transmission were acquired by cell culture.the androgen receptor agonist DHT and the androgen receptor antagonist FLU applied to the culture prostate cancer cell line of androgen at different concentrations drugs.Mitochondria at different time points of LNCaP under the interventions were extracted.Mitochondrial radidal and differences at different time points and under the action of different drug concentration in LNCaP were measured and compared.Results After DHT acted on LNCaP cells, the GSH of mitochondron decreased significantly（P﹤0.05）, but the difference of the decreasing degree of each index in different time points was found.After FLU acted on LNCaP cells, the content of GSH increased significantly on the middle dose group（P﹤0.05）,the other groups was not significantly（P〉0.05）.Conclusions DHT can cause decreasing of GSH on the mitochondrion,and promote cell proliferation.FLU can not remove the GSH on LNCaP cells,and cause the accumulation of free radicals, can inhibit the proliferation of LNCaP cells effectively.