PTTG1蛋白表达与骨肉瘤患者临床病理特征及预后的相关性

Correlation Analysis of PTTG1 Protein Expression with the Clinical Features and Prognosis of Osteosarcoma

目的探讨垂体肿瘤转化基因1(PTTG1)蛋白在骨肉瘤(OS)中的表达及其与预后的相关性。方法采用免疫组化法检测76例骨肉瘤组织、50例骨软骨瘤和50例正常骨组织中PTTG1蛋白的表达,对骨肉瘤患者随访9~60个月,分析PTTG1蛋白的表达及其与预后的相关性。结果相比正常骨组织以及骨软骨瘤组织,骨肉瘤组织中PTTG1蛋白的表达水平明显增高(P<0.05)。PTTG1蛋白的表达与临床分期、术后复发、转移和3年生存期有关(P<0.05),与年龄、性别、病理类型、病变部位无关(P>0.05)。PTTG1蛋白阳性表达患者的生存率均显著低于PTTG1阴性表达患者(P<0.05)。临床分期、转移和PTTG1蛋白表达是影响骨肉瘤患者生存率的独立危险因素(P<0.05)。结论 PTTG1蛋白的表达上调与骨肉瘤的发生发展相关,PTTG1蛋白阳性表达是骨肉瘤患者的不良预后因素。

Objective To investigate the PTTG1 （Pituitary tumor transforming gene 1） protein expression in osteosarcoma and its cor- relation with the clinical features and prognosis. Methods Immunohistochemistry method was conducted to detect the protein expression of PTTG1 in 76 cancer tissues from osteosarcoma patients, 50 osteochondroma tissues and 50 normal bone tissues. Furthermore, all patients were followed-up for 9-60 months. Analyzed the correlation of PTTG1 protein expression with the prognosis of osteosarcoma. Results The protein expression of PTTG1 was significantly higher in cancer tissues than in osteochondroma tissues and in normal bone tissues. Besides, PTITG1 protein expression was also measured higher in osteochondroma tissues than in normal bone tissues （P〈0.05）. In addition, PTTG1 protein expression showed association with clinical features of osteosarcoma, including clinical stage, postoperative recurrence, metastasis and 3-year survival time （P〈 0.05）, whereas no correlation was found with age, gender, pathological type and involved region （P〉 0.05）. Fur- thermore, the survival rate of the group with positive PTTG1 protein expression was significantly lower than that of the group with negative PTTG1 protein expression （P 〈 0.05）. Clinical stage, metastasis and PTTG1 protein expression were independent risk factors for the survival rate of patients with osteosarcoma. Conclusion Protein expression of PTI＇G1 had correlation with the occurrence and development of oste- osarcoma. Positive expression of PTTG1 may be the poor prognostic factor of osteosarcoma.