人肝癌细胞自分泌促性腺激素释放激素的形态学证据

Morphological evidence of autocrine of gonadotropin releasing hormone by human hepatocarcinoma cells

目的 :明确肝癌组织细胞是否具有分泌促性腺激素释放激素 (GnRH)的功能。 方法 :采用免疫组化和原位杂交技术 ,研究人肝癌组织中GnRH及其受体的表达。 结果 :10例石蜡包埋的肝细胞癌 (HCC)组织中有 7例表现为GnRH及其受体蛋白免疫反应阳性 ,GnRH及其受体均定位于肝癌细胞的胞膜和胞质 ;4例新鲜肝癌组织中有 2例检测出GnRH mRNA ,阳性杂交信号定位于肝癌细胞的胞质 ,胞核未见杂交信号。 结论 :本研究表明 ,肝癌组织细胞具有自分泌GnRH的功能 。

Objectives:To clarify whether the hepatocellular carcinoma (HCC) cells have the ability of autocrine gonadotropin releasing hormone(GnRH). Methods:The expression of GnRH and its receptor(GnRH-R) were detected in HCC by immunohistochemistry and in situ hybridization techniques. Results:Immunoreactivity for GnRH and its receptor protein was observed in 7 of 10 cases of paraffin-embedding tissues of human HCC,and the positive signal was located in the cytoplasm and the membrane of hepatoma cells;GnRH-mRNA was detected in 2 of 4 cases of fresh surgical resected HCC tissues,hybridization signals were found mainly in the cytoplasm of cancer cell,with no positive signal was revealed in the nucleus. Conclusions:These results suggested that some human HCC cells could autocrine GnRH.The present study provided the theoretical basis for further investigating the relationship between GnRH and biological characteristics of human hepatocarcinoma.