甘露糖受体介导的人参多糖注射液对肝癌细胞增殖抑制

The Mannose Receptor Mediated Ginseng Polysaccharide Injection on Iiver Cancer Cell Proliferation Inhibition

目的探讨人参多糖注射液对人肝癌细胞BEL-7402和Hpe G2增殖抑制的机制及与甘露糖受体表达水平的关系。方法实验于2014年6月在齐齐哈尔医学院中心研究室进行,利用荧光免疫法检查甘露糖受体在人肝癌细胞BEL-7402和Hpe G2上的表达水平;采用CCK8检测细胞的增殖抑制情况。结果免疫荧光结果 ：甘露糖受体在人肝癌细胞Hpe G2上表达分布较少,而在人肝癌细胞BEL-7402上表达分布较多;CCK8结果：在一定浓度范围内,人参多糖注射液对人肝癌细胞BEL-7402和Hpe G2的增殖有明显抑制作用（P〈0.05）,并且与浓度和时间呈正相关,同时证明人参多糖注射液对人肝癌细胞BEL-7402的增殖抑制作用明显高于对Hpe G2增殖抑制作用（P〈0.05）。结论人参多糖注射液对人肝癌细胞BEL-7402和Hpe G2的增殖有明显的抑制作用;对人肝癌细胞BEL-7402的增殖抑制作用高于对Hpe G2的抑制作用,增殖抑制作用的高低与甘露糖受体表达水平呈正相关。

Objective To study the mechanism of ginseng polysaccharide injection on the proliferative inhibition of hepatoma cell line BEL-7402 and Hpe G2 as well as the relationship between the ginseng polysaccharide injection and expression level of mannose receptor. Methods Experiment was carried out in the laboratory center of Qiqihar Medical School in June 2014,Fluorescence immunoassay was used to detect the expression levels of mannose receptor in hepatoma cell line BEL-7402 and Hpe G2. Cholecystokinin octapeptide(CCK8) was used to observe proliferative inhibition of cells. Results By immunofluorescence, it was detected that the weak expression and distribution of the mannose receptor in hepatoma cell line Hpe G2, but high expression and distribution in BEL-7402. By CCK8, ginseng polysaccharide injection could effectively inhibit the proliferations of BEL-7402 and Hpe G2 within certain concentration range(P<0.05), which was positively correlated with concentration and time. And there was more significant anti-proliferative effect on BEL-7402 than Hpe G2(P<0.05).Conclusion Ginseng polysaccharide injection could effectively inhibit the proliferations of hepatoma cell line BEL-7402 and Hpe G2. Anti-proliferative effect on BEL-7402 is more significant than Hpe G2. The anti-proliferative effect is positively correlated with expression of the mannose receptor.