鸭源鸡杆菌外膜蛋白A基因克隆及结构与功能分析

Cloning of the Outer Membrane Protein A Gene of Gallibacterium anatis and Analysis of Its Structure and Function

为筛选鸭源鸡杆菌(G.anatis)潜在的保护性抗原基因,参考Gen Bank中G.anatis UMN179的外膜蛋白A(OmpA)基因序列设计1对引物,对鸭源鸡杆菌PDS-RZ-1-SLG(RZ)株的OmpA基因进行克隆,并应用相关软件对其核苷酸及氨基酸序列进行生物信息学分析。结果显示,OmpA基因大小为1 083 bp,编码360个氨基酸;鸭源鸡杆菌RZ株的OmpA与UMN179、F149及12656/12的OmpA氨基酸相似性分别为93.2%、95.2%、92.7%;OmpA蛋白分子质量为38.4 ku,等电点为6.77,具有稳定性,N端有1个疏水性的α螺旋信号肽。

To screen potential protective antigen genes for developing genetic engineering vaccine of Gal-libacterium anat is, a pair of primers were designed according to G. anatis UMN179 OmpA gene sequence published in Genbank,and the OmpA gene of G. anatis PDS-RZ-l-SLG was cloned and sequenced. In ad -dition, the bioinformatics analyses of OmpA nucleotide and amino acid sequences were made with bioinfor-matics tools and online servers. The results showed that the OmpA gene was 1 083 bp in s iz e , encoding 360 amino acid residues. Compared with the amino acid sequences of the OmpA of G. anatis UMN179,F149 and 12656/12,the similarity were 93. 2% ,95. 2% and 92. 7% , respectively. OmpA is a stable protein, with a relative molecular weight of 38.4 ku and a isoelectric point of 6. 77 ,containing a hydrophobic lead-er peptide region,which is likely to consist of a long stretch of a helical structures in N-terminal end.