玉米ZmCIPK23基因Mutator插入突变体的鉴定

Identification of the Mutator Insertional Mutants in ZmCIPK23

【目的】玉米ZmCIPK23基因的候选突变体连续与玉米自交系B73回交并自交,最终得到以B73为背景的纯合突变体,为研究该基因的功能奠定材料基础。【方法】从玉米Mutator(Mu)突变体库中定向筛选到玉米ZmCIPK23基因的候选突变体。在田间种植候选突变体,利用巢式PCR对这些后代植株进行鉴定,选择阳性植株与B73杂交。通过连续的回交及自交,最终获得该基因的纯合突变体。【结果】通过PCR鉴定,获得了以B73为背景的ZmCIPK23基因纯合的突变体zmcipk23。【结论】获得了玉米Zm CIPK23基因的Mu插入的纯合突变体zmcipk23,为研究该基因的功能奠定了基础。

【 Objective】 The candidate mutants of maize gene were continuously backcrossedand self - pollinated with B73 in fields, and finally homozygous mutants with B73 background were obtained, which laid the material foundation for studying the function of this gene.【 Method] The candidate mutants of ZmCIPK23 were screened from a maize Mutant library. They were planted and analyzed by nested PCR. The positive plants with Mu - insertion in the ZmCIPK23 gene were self - pollinated and backcrossed with B73 in the field. Thus the homozygous mutants were obtained.【 Result】 The homozygous mutant of , ZV tiC/PKS gene was obtained by PCR.【 Conclusion】The homozygous mutant （zmcipk23） of Mu insertion of , ZV tiC/PKS gene was obtained. This study has provided a foundation for the functional analysis of the ZmCIPK23 gene.