抗性和敏感生物型菵草代谢精噁唑禾草灵的速度差异

Differences in Metabolism Rate of Fenoxaprop-P-Ethyl Among Beckmannia syzigachne Biotypes

为了测定菵草抗性生物型SD-04-SS和敏感生物型SD-12对精噁唑禾草灵代谢速度的差异,建立了精噁唑禾草灵及其主要代谢产物精噁唑禾草酸、6-氯苯并噁唑酮的高效液相色谱(HPLC)分析方法。结果表明,在0.123~30.000μg/m L范围内、0.977~1 000.000μg/m L范围内、0.469~30.000μg/m L范围内6-氯苯并噁唑酮、精噁唑禾草酸、精噁唑禾草灵浓度与响应值线性关系良好,相关系数(r)分别为0.991 2、0.972 2、0.996 3。应用上述方法分析发现,精噁唑禾草灵处理后2 h在菵草体内即可检测到精噁唑禾草酸和6-氯苯并噁唑酮,药后2~96 h抗性生物型SD-04-SS体内精噁唑禾草灵的含量始终略高于敏感生物型SD-12,药后24~72 h抗性生物型SD-04-SS体内精噁唑禾草酸的含量显著低于敏感生物型SD-12,说明抗性生物型降解精噁唑禾草酸的速度比敏感生物型更快。

An HPLC method was developed to analyze the concentration of fenoxaprop-P-ethyl,fenoxaprop-P and 6-chlorobenzoxazolone in Beckmannia syzigachne,and to determine differences in the rate of fenoxaprop-P-ethyl degradation between the resistant biotype SD-04-SS and susceptible biotype SD-12. Excellent linearity was obtained in the range of 0. 123-30 μg/m L,0. 977-1000 μg/m L and 0. 469-30 μg/m L for 6-chlorobenzoxazolone,fenoxaprop-P and fenoxaprop-P-ethyl,respectively,with correlation coefficient of 0. 9912,0. 9722 and 0. 9963,respectively.The method was employed to analyze the concentration of fenoxaprop-P-ethyl,fenoxaprop-P and 6-chlorobenzoxazolone in both biotypes. Fenoxaprop-P and 6-chlorobenzoxazolone could be detected in B. syzigachne 2 hours after treatment with fenoxaprop-P-ethyl. The concentration of fenoxaprop-P-ethyl in SD-04-SS（R） was always higher than that in SD-12（S） within 2 - 96 hours after treatment（HAT）,but the concentration of fenoxaprop-P in SD-04-SS（R）was always higher than that in SD-12（S）within 24-72 hours.Thefore the resistant biotype SD-04-SS degrades fenoxapropP faster than the susceptible biotype SD-12.