GLUT4和脂联素在大鼠非酒精性脂肪性肝病中的作用

The Role of GLUT4 and Adiponectin in Nonalcoholic Fatty Liver Disease

目的探讨GLUT4和脂联素在大鼠非酒精性脂肪性肝病中的作用。方法 2015年6月—2015年11月选用48只SD大鼠一半雌鼠一半雄鼠,雌鼠雄鼠随机分为对照组与实验组均为24只。对照组为正常,实验组为高脂饮食组。每组又分为3、6、9、12周4组按时间点处死各组大鼠,测定肝组织中GLUT4、血清中的脂联素、空腹血糖。结果肝组织中GLUT4、血清中的脂联素在脂肪性肝病的形成过程中含量不断减少,肝组织中GLUT4的浓度由对照组(3.65±0.08),3、6、9、12周逐渐降低分别为(3.32±0.07)、(3.13±0.11)、(2.96±0.08)、(2.31±0.19)血清中的脂联素由对照组(4.22±0.43),3、6、9、12周逐渐降低分别为(4.18±0.26)、(3.90±0.27)、(3.96±0.21)、(4.07±0.24)。而且与肝脂肪变程度呈负相关,血糖呈正相关。结论实验组肝组织中GLUT4、血清中的脂联素相比对照组明显减少,表明CLUT4与脂联素在非酒精性脂肪性肝病中有一定作用。

Objective To investigate the role of GLUT4 and adiponectin in nonalcoholic fatty liver disease. Methods48 SD rats with half male half female, male female rats were randomly divided into control group and experimental group were 24. Normal control group, the experimental group was high-fat diet group. Each group was divided into 3weeks, 6 weeks, 9 weeks, 12 Thursday set point in time the rats were sacrificed to measure liver tissue GLUT4, serum adiponectin, fasting blood sugar. Results In liver tissue in GLUT4 and serum adiponectin in fatty liver formation content decreased constantly, concentration of GLUT4 in liver tissue from control group 3.65 ± 0.08, 3 weeks, 6 weeks, 9weeks and 12 weeks decreased gradually were（3.32 ±0.07）,（0.11 ± 3.13）,（2.96 ± 0.08）,（2.31 ± 0.19） serum adiponectin comprised the control group（4.22 ± 0.43）, 3 weeks, 6 weeks, 9 weeks and 12 weeks decreased gradually（4.18 ± 0.26）,（3.90 ± 0.27）,（3.96 ± 0.21）,（4.07 ± 0.24）, respectively. It was negatively correlated with the degree of hepatic steatosis, and the blood glucose was positively correlated. Conclusion The hepatic tissue GLUT4, serum adiponectin was significantly reduced compared to the control group, suggesting that adiponectin CLUT4 and have a role in nonalcoholic fatty liver disease.