东南沿海地区1980-2015年汉坦病毒分子特征及流行病学分析

The molecular characteristics and epidemiological analysis of Hantavirus in southeast coastal area of China from 1980 to 2015

目的探讨1980-2015年从东南沿海地区不同地域、时间、宿主、媒介及患者中分离的汉坦病毒(HV)的遗传进化及流行规律。方法运用遗传流行病学、分子流行病学和生物信息学方法分析1980-2015年东南沿海地区(江苏、浙江、福建省和上海市)HV的S、M片段高变区基因的变异位点与频率,结合HV、宿主、媒介与生态环境4个方面及流行病学资料,分析HV在不同地域、时间、自然宿主以及重要传播媒介之间的演变规律。结果东南沿海地区肾综合征出血热(HFRS)临床患者感染的病毒以汉滩型(HTN)HV居多,主要与76-118株同源为主,汉城型病毒(SEOV)与Z37株相似的病毒为主;为HV HTN和SEO的混合型疫区,呈现高度的地理聚集现象;鼠类携带HV以SEOV为主,病毒分支与携带宿主的种类和采集地点有关,SEOV主要来源于褐家鼠,HTNV主要来源于黑线姬鼠。结论 HV和HFRS疫区的形成和维持具有一定的内在规律。

Objective To analyze the genetic evolution and epidemiological pattern of Hantavirus（HV）isolated fromdifferent hosts, vectors and patients in different time from different regions of southeastern China from 1980 to 2015.Methods The mutation site and frequency in S, M segment hypervariable region of HV isolated from southeastern China,including Jiangsu province, Zhejiang province, Shanghai and Fujian province, from 1980 to 2015 were detected. Combiningthe information of HV, hosts, vectors, environment and epidemiology, the HV evolution laws in different regions, time,natural reservoir and important vectors were analyzed.Results The HV strains isolated from the hemorrhagic fever withrenal syndrome（HFRS）patients in the southeast coastal area were mainly the Hantaan virus（HTNV）, which were highlyhomologous with the 76-118 strain. While the Seoul virus（SEOV）carried by patients exhibited the highest sequencesimilarity to the Z37 strain. The southeast coastal area is the mixed type of HV-affected area, with apparent endemicity. TheHV isolated from the rodents shared the highest identity to SEOV. The HV clades were related to host types and thelocations of sample collection. SEOV was carried primarily by Rattus norvegicus, while HTNV was carried primarily byApodemus agrarius.Conclusion The formation and maintenance of HFRS epidemic areas show a certain regularity.