2016年江门市白纹伊蚊对常用杀虫剂的抗药性调查

Resistance of Aedes albopictus to commonly used insecticides in Jiangmen city, Guangdong province, 2016

目的调查江门市白纹伊蚊幼虫和成蚊对5种常用杀虫剂的抗药性情况。方法采用WHO推荐的蚊虫抗药性生物学测定方法,即成蚊采用接触筒法,蚊幼虫采用浸渍法;以实验室饲养的敏感试虫为参考品系,分别测定2016年5-10月采自野外的成蚊和蚊幼虫的抗药性。结果 7个白纹伊蚊幼虫种群对双硫磷、残杀威、溴氰菊酯、高效氯氰菊酯、氯菊酯的半数致死浓度(LC_(50))分别为0.001 8~0.005 7、1.031 9~2.778 5、0.003 8~0.015 0、0.004 3~0.025 4和0.002 1~0.027 9 mg/L,为敏感品系的0.7~2.3、1.5~3.9、4.2~16.7、3.3~19.5和0.4~5.0倍;7个白纹伊蚊成蚊种群对马拉硫磷、残杀威、氯菊酯为敏感或可疑抗性。江海区、新会区和鹤山市白纹伊蚊成蚊种群对溴氰菊酯产生抗性,死亡率分别为75.93%、64.15%和78.69%;蓬江区和恩平市白纹伊蚊成蚊种群对高效氯氰菊酯产生抗性,死亡率分别为77.19%和73.42%。结论江门市白纹伊蚊幼虫种群对双硫磷均为敏感,对残杀威和拟除虫菊酯类杀虫剂产生不同程度的抗性;成蚊对拟除虫菊酯类产生抗性或可疑抗性。

Objective To investigate the resistance in larvae and adults of Aedes albopictus to five commonly used insecticides in Jiangmen city, Guangdong province, China in 2016. Methods WHO recommended methods-bioassays were performed on the collected Ae. albopictus. With reference to the laboratory susceptible strain, insecticides resistance was tested with exposure tubes on adults and dipping method on larvae. Results The LCs0 values of temephos, propoxur, deltamethrin, beta-cypermethrin, and permethrin for 7 populations of Ae. albopictus larvae were 0.001 8-0.005 7, 1.031 9-2.778 5, 0.003 8-0.015 0, 0.004 3-0.025 4, and 0.002 1-0.027 9 mg/L, respectively （resistant ratio 0.7-2.3, 1.5-3.9, 4.2-16.7, 3.3-19.5, and 0.4-5.0, respectively）. Seven populations of adult mosquitoes can be considered susceptible or probable resistance to malathion, propoxur and permethrin. Three populations of adult Ae. albopictus （Jianghai district, Xinhui district and Heshan city） were resistant to dehamethrin （mortality 75.93%, 64.15%, and 78.69%）. Two populations of adult Ae. albopictus （Pengjiang district and Enping city） were resistant to beta-cypermethrin （mortality 77.19% and 73.42%）. Conclusion The larvae ofAe. albopictus in Jiangmen city are susceptible to temephos and have developed different levels of insecticide resistance to propoxur and pyrethroids. Adult mosquitoes showed remarkable resistance or probable resistance to pyrethroids.