基于石墨烯量子点构建生物传感器检测尿酸和多巴胺的实验研究

Simultaneous Electrochemical Detection of Uric Acid and Dopamine Based on Graphene Quantum Dot Modified Glassy Carbon Electrode

目的制备石墨烯量子点(GQDs)以构建生物传感器检测尿酸和多巴胺。方法一步法碳化构橼酸合成含羧基的石墨烯量子点用以构建电化学生物传感器,以差分脉冲法检测尿酸和多巴胺,并评估其检测性能。采集2016年7月29日三名实验人员晨尿,用构建的传感器以完成生物样本检测。结果透射电镜观察到制备的GQDs大小在3~5 nm之间。构建的生物传感器检测50~600μmol/L尿酸(r^2=0.996 6)和2~240μmol/L多巴胺(r^2=0.992 5)的浓度线性相关性良好。检测尿液样本中尿酸的浓度(n=3),RSD值小于3%。尿酸和多巴胺的加标回收率范围分别在95.7%~101.4%和96%~102%。结论成功构建了基于石墨烯量子点的生物传感器检测尿酸和多巴胺。

Objeetive To preparegraphene quantum dots （GQDs） and construct a novel biosensor for determination of dopa- mine （DA） and uric acid （UA）. Methods The GQDs was prepared by carbonization method from citric acid as carbon sources. Differential pulse voltammetry was used by the modified electrodes to detect uric acid and dopamine,and the detec- tion performance was evaluate. Collected three experimenters morning urine on July 29,2016. The proposed sensor was used for biological samples detection. Results The size of GQDs were between 3 to 5 nm,which was observed by transmission e- lectron microscopy （TEM）. The proposed sensor had good linear correlation of 50-600μmol/L UA （r2=0. 996 6） and 2- 240 μmol/L DA （r2=0. 992 5）. In uric acid in urine samples＇ detection （n=3）, RSD value was less than 3%. The standard addition recovery rates of UA and DA were in 95.7%-101.4% and 96μ-102μ respectively. Conclusion The biosensor based on GQDs for determination DA and UA had been constructed successfully.