双阴性调节性T细胞(DN Tregs)在系统性红斑狼疮患者外周血中的变化及意义

Change and Clinical Significance of Peripheral Blood DN Tregs in Patients with Systemic Lupus Erythematosus

目的研究系统性红斑狼疮(systemic lupus erythematosus,SLE)患者外周血中TCRαβ^+CD3^+CD4^-CD8^-T细胞即双阴性调节性T细胞(double negative regulatory T cells,DN Tregs)的表达水平及功能,并探讨DN Tregs在SLE疾病进程中的作用。方法通过流式细胞术检测20例SLE患者及20例健康对照者外周血CD3^+TCRαβ^+细胞中DN Tregs细胞的比例;采用ELISA的方法检测SILE患者及健康对照者血浆中IFN-γ,TNF-α,IL-6和IL-17A的表达水平,并分析DN Tregs与各细胞因子的相关性。结果与健康对照组相比,SLE患者组DN Tregs占TCRαβ^+细胞的比例升高,两组之间差异有统计学意义(t=3.54,P<0.01),SLE患者血浆中IFN-γ,TNF-α和L-6水平高于对照组,两组之间差异均具有统计学意义(t=2.824,2.085,2.304,均P<0.05)。DN Tregs的水平与IFN-γ的含量呈正相关(r=0.52,P=0.02),而与TNF-α,IL-6的含量无相关性(r值分别为0.17和0.16,P值分别为0.47和0.49)。结论 SLE患者外周血DN Tregs细胞比例显著上升,DN Tregs细胞可能在SLE发病及进展中发挥重要作用。

Objective To study the expression and function of TCRαβ＋ CD3＋ CD4- CD8- T （Double negative regulatory T cells,DN Tregs） in peripheral blood of Systemic lupus erythematosus （SLE） patients, and investigate their function in path- ogenesis of SLE. Methods TCRαβ3＋ CD4- CD8- T cells were quantified as percentage of total CD3＋ T cells in peripheral blood from 20 SLE patients and 20 healthy controls by flow cytometry. Used ELISA to test the cytokine levels （IFN-γ, TNF-α, IL-6, IL-17A） in the plasma of SLE patients. And analyzed the relationship between the percentage of DN Tregs and cytokines levels. Results The ratio of DN Tregs in TCRαβ＋ T cells was significantly increased in SLE patients compared to healthy donors （t= 3.54, P〈0. 01）. The levels of IFN-γ, TNF-α and IL-6 in plasma of SLE patients were higher than healthy donors （t=2. 824,2. 085,2. 304,P〈0.05）. The frequency of DN Tregs was found to correlate with IFN-γ （r= 0.52 ,P=0.02） but not TNF-α （r=0.17 ,P=0.16） and IL-6 （r=0.47,P=0.49）. Conclusion This study reveals frequen- cy of DN Tregs in peripheral blood of SLE patients was higher than healthy controls, the frequency of DN Tregs was also found to correlate with IFN-γ levels,which means that DN Tregs may play an important role in pathogenesis of SLE.