结肠癌组织中microRNA-126表达及临床意义

Expression of microRNA-126 in Colon Cancer and Its Clinical Significance

目的探讨microRNA-126在结肠癌组织中的表达及生物学意义。方法收集104例结肠癌手术患者,实时定量聚合酶链反应(RT-PCR)检测结肠癌患者的结肠癌组织以及癌旁组织中microRNA-126的表达(共104对)。应用慢病毒表达载体构建microRNA-126细胞系,并进一步通过体外实验研究microRNA-126对结肠癌细胞增殖、迁移和侵袭能力的影响。结果结肠癌组织中microRNA-126相对表达水平(0.63±0.11)明显低于癌旁组织(1.08±0.15),差异有统计学意义(t=14.561,P<0.01)。结肠癌组织中microRNA-126表达阳性率(61.5%)显著低于癌旁组织(86.5%),差异有统计学意义(X^2=16.908,P<0.05)。microRNA-126表达与结肠癌Dukes分期、浸润深度、有无淋巴结转移及远处转移明显相关(P<0.05)。在有基质胶的Transwell实验中,转染组细胞迁移数(11.26±4.85个)明显低于空白对照组(264.37±32.15个),差异有统计学意义(t=23.418,P<0.01)。无基质胶的Transwell实验中,转染组细胞迁移数(83.75±13.74个)也明显低于空白对照组(339.64±26.38个),差异有统计学意义(t=12.682,P<0.01)。microRNA-126可以抑制SW480细胞的增殖及其侵袭转移。结论 microRNA-126可明显抑制结肠癌细胞的发生发展,影响结肠癌细胞的生物学行为。

Objective To investigate the expression and biological significance of microRNA-126 in colon cancer tissue. Meth- ods The expression of microRNA-126 in colon cancer tissues and adiaeent tissues of colon cancer patients was detected by real-time quantitative polymerase chain reaction （RT-PCR） in 104 patients with colon cancer surgery （total of 104 pairs）. The lentiviral vector was used to construct microRNA-126 ceil line,and the effects of microRNA-126 on proliferation,migra- tion and invasion of colon cancer cells were further studied in vitro. Results The relative expression of mieroRNA-126 in colon cancer tissues （0.63±0.11） was significantly lower than that in adjacent tissues （1.08±0.15）,the difference was sta- tistically significant（t=14. 561 ,P〈0.01）. The positive rate of microRNA-126 expression in colon cancer tissues （61.5 % ） was significantly lower than that in adjacent cancer tissues （86.5 %） the difference was statistically significant（x^2 = 16. 908, P〈0.05）. The expression of microRNA-126 was significantly correlated with Dukes stage,depth of invasion, lymph node metastasis and distant metastasis （P〈0.05）. In the Transwell experiment with matrix glue,the number of cell migration in transfection group （11.26±4.85） was significantly lower than that in blank control group （264.37±32.15）,the difference was statistically significant （t= 23. 418, P〈0.01）. In the Transwell experiment without matrix glue, the number of cell mi gration in transfection group （83.75±13.74） was significantly lower than that in blank control group （339.64 ± 26.38）, the difference was statistically significant（t= 12. 682, P〈0.01）. MicroRNA-126 could inhibit the proliferation and invasion of SW480 ceils. Conclusion MicroRNA-126 can significantly inhibit the development of colon cancer cells and affect the bio logical behavior of colon cancer cells.