口腔鳞状细胞癌患者血清中CCL2和VEGF表达的初步研究

Preliminary Study on the Expression of CCL2 and VEGF in Serum of Patients with Oral Squamous Cell Carcinoma

目的探讨口腔鳞状细胞癌(OSCC)患者血清中单核细胞趋化蛋白-1(MCP-1/CCL2)及血管内皮生长因子(VEGF)的表达及意义。方法收集27例健康对照者和85例OSCC患者的血清,用ELISA方法检测CCL2及VEGF的表达水平,分析两者相关性及与临床病理参数的关系。结果 OSCC患者血清CCL2水平为69.12±19.54 pg/ml,显著低于健康对照者(103.41±34.42 pg/ml),差异具有统计学意义(t=6.477,P<0.05)。OSCC患者血清CCL2水平与TNM分期明显相关(t=2.193,P<0.05)。OSCC患者血清VEGF水平为145.76±49.34 pg/ml,显著高于健康对照者(70.35±14.93 pg/ml),差异具有统计学意义(t=3.92,P<0.05)。CCL2水平与VEGF水平呈负相关关系(r=-0.216,P<0.05)。受试者工作特征曲线(ROC曲线)显示血清中CCL2水平及CCL2/VEGF比值都能够很好地区分健康人和OSCC患者(P<0.05),其最佳临界值分别为98.61 pg/ml及0.82。结论 OSCC患者血清中CCL2表达降低,VEGF表达增高,提示其可能与OSCC发生、发展相关。检测血清CCL2及VEGF水平可以作为OSCC的筛查指标。

Objective To study the expression and significance of monocyte chemotactic protein-1 （MCP-1/CCL2） and vascu- lar endothelial growth factor （VEGF） in serum samples of oral squamous cell carcinoma （OSCC） patients. Methods The concentrations of CCL2 and VEGF in the serum was assessed by ELISA in healthy donors （n=27） and OSCC patients （n= 85）. Then analyzed the correlation between the concentrations of CCL2 and VEGF and the relationship with patients＇ clini- copathological characteristics. Results CCL2 concentration was lower in OSCC patients than in healthy donors （69.12 ± 19.54 pg/ml Vs 103.41 ± 34.42 pg/ml, t = 6. 477, P〈0.05）. The expression of CCL2 was positively associated to TNM stage in OSCC （t= 2. 193,P〈0.05）. VEGF concentration was higher in OSCC patients than in healthy donors （145.76± 49.34 pg/ml vs 70.35d：14.93 pg/ml,t=3.92,P〈0.05）. There was a negative correlation between CCL2 and VEGF （r= -0. 216,P〈0.05）. The receiver operating characteristic （ROC） curve suggests that CCL2 and CCL2/VEGF in serum are good diagnostic markers to discriminate healthy people from OSCC patients, the cutoff values was 98.61 pg/ml and 0.82. Conclusion The expression of CCL2 and VEGF in serum correlated to OSCC progression,and it can be a potential diagnostic biomarker for oral disease.