支气管哮喘患儿血清MMP-9,Gal-3和TGF-β1与肺功能的关系研究

Relationship between Serum MMP-9,Gal-3,TGF-β1 and Pulmonary Function in Children with Bronchial Asthma

目的分析支气管哮喘患儿血清基质金属蛋白酶-9(MMP-9),半乳糖凝集素-3(Gal-3),转化生长因子-β1(TGF-β1)与肺功能的关系。方法选取深圳市龙华新区人民医院儿科门诊2012年3月1日~2016年8月31日之间的儿童218例作为研究对象,通过血清学检验MM-9,Gal-3,(TGF-β1)的水平,再使用肺功能检验应用测定仪器,检查所有儿童的肺功能,指标选择FEV_1%,FEV_1/FVC和FEF_(50),分别比较对照组、轻度组和中重度组儿童以上指标的数据。结果对三组218例儿童的血清中MM-9,Gal-3和TGF-β1水平进行检测,将获得的数据进行方差分析检验,结果差异有统计学意义(F=35.34,46.51,56.73,均P<0.05),经过q检验,任意两组之间的数据差异也有统计学意义,对三组218例儿童的第一秒用力呼气量/1秒用力呼气量预测值的百分比(FEV_1%)、1秒用力呼气量/用力肺活量百分比(FEV_1/FVC)、用力呼气50%肺活量的瞬间流量(FEF_(50))进行检测,将获得的数据进行方差分析检验,结果差异均有统计学意义(F=78.13,52.69,26.24,均P<0.05),经过q检验,任意两组之间的数据也同样出现统计学差异。结论血清MM-9,Gal-3,TGF-β1三种细胞因子与支气管哮喘发作程度和患儿肺功能下降程度有着密切联系。

Objective To study the relationship between serum MMP-9, Gal-3, TGF-β1 and lung function in children with bronchial asthma. Methods The serum levels of matrix metalloproteinase-9 （MMP-9）, serum galectin-3 （MMP-9）, serum metalloproteinase-3 （MMP 9） and serum albumin were measured in 218 pediatric children between March 1,2012 and Au- gust 31,2016 in Shenzhen Longhua New District People＇s Hospital pediatric outpatient clinic. FEV1 %, FEV1/FVC and FEFs0 were measured and compared respectively with the index data of the control group. The lung function of the children was significantly higher than that of the control group （P〈0.05）. Mild group and severe group children above. Results Serum levels measured in 218 children （F=35.34,46.51,56.73,all P〈0.05）. The data of any two groups also showed statis- tical difference after q test, and the difference was statistically significant （FEV1 %）, 1-second forced expiratory volume/ forced vital capacity percentage （FEV1/FVC） ,forced expiratory 50%（FEF50 ） of the first-second forced expiratory volume/ forced expiratory volume of 1 second （F=78.13,52.69,26.24,all P〈0.05）. After the q test,the data of any two groups were analyzed by ANOVA. Between the data were also statistically significant differences. Conclusion MM-9, Gal-3 and TGF-1β in bronchoalveolar lavage and the degree of pulmonary function decline have close ties.