UHRF1检测在良恶性胸腔积液鉴别诊断中的研究

Study on the Detection of UHRF1 in the Differential Diagnosis of Benign and Malignant Pleural Effusion

目的探讨泛素样含PHD和环指域1(ubiquitin-like with PHD and ringfinger domains 1,UHRF1)在良恶性胸腔积液中的表达及临床意义。方法收集南京市江宁医院2015年11月~2017年2月入院的130例胸腔积液患者,其中肺癌引起的恶性胸腔积液54例,肺部良性病变胸腔积液76例。肺部良性病变胸腔积液中,结核性胸腔积液36例,炎症性胸腔积液40例。采用酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)分别检测各组UHRF1水平,并分析其与临床病理的关系。结果肺癌患者胸腔积液UHRF1水平(437.28±140.05 ng/L)高于肺部良性病变者(338.47±163.72ng/L),两组比较差异有统计学意义(t=3.596,P=0.0005)。非腺癌患者UHRF1水平(470.59±127.74ng/L)高于腺癌患者(377.78±145.81ng/L),差异有统计学意义(t=2.329,P=0.023)。良性病变组中结核性胸腔积液UHRF1水平为365.51±167.54 ng/L,与炎性胸腔积液(314.13±158.34 ng/L)相比较,差异无统计学差异(t=1.374,P=0.174)。良恶性胸腔积液UHRF1水平均与年龄、性别及吸烟史无关。结论 UHRF1是诊断良恶性胸腔积液一个潜在指标。

Objective To investigate the expression and clinical significance of ubiquitin-tike with PHD and ringfinger domains I（UHRF1） in benign and malignant pleural effusion. Methods From November,2015 to February,2017,130 cases of pleu- ral effusion were collected in Jiangning hospital of Nanjing,including 54 cases of malignant pleural effusion caused by lung cancer and 76 cases of lung benign pleural effusion. And the latter contained 36 cases of tuberculous pleural effusion and 40 cases of inflammatory pieural effusion. Enzymeqinked immunosorbent assay（ELISA） were used to detect the level of UHRF1 in each group, and the relationship between UHRF1 and clinical pathology was analyzed. Results The level of UHRF1 437.28% 140.05 ng/L in patients with lung cancer was higher than that 338.47-7163.72 ng/L in patients with be- nign lung diseases, and the differences were statistically significant （t= 3. 596, P = 0. 0005）. The level of UHRF1 470.59 ± 127.74 ng/L in patients with non- adenocarcinoma was higher than that 377.78±145.81 ng/L in patients with adenocarci- noma, and the differences were statistically significant（t= 2. 329, P= 0. 023）. In group with benign lesion, there were no sig- nificant differences（t= 1. 374, P= 0. 174） between the level of UHRF1 365.51± 167.54 ng/L in tuberculous pleural effusion and the level of UHRF1 314. 13±158. 34 ng/L in inflammatory pleural effusion. And the level of UHRF1 in benign and ma- lignant pleural effusion was not related to age,sexuality and smoking history. Conclusion UHRF1 is a potential marker in the differential diagnosis of benign and malignant pleural effusion.