寻常型天疱疮患者血清HSV,CMV和EBV抗体IgG检测的临床意义

Clinical Significance of the Levels of IgG Antibodies Against HSV,CMV and EBV in Patients' Serum with Pemphigus Vulgaris

目的通过评价和比较在寻常型天疱疮患者和健康人抗单纯疱疹病毒1型和2型(HSV1和HSV2),抗巨细胞病毒(CMV)和抗EB病毒(EBV)抗体IgG的水平,研究病毒特别是疱疹病毒在寻常型天疱疮中的意义。方法 23例寻常型天疱疮和26例健康人组成的实验组和对照组,分别从两组中取血清样品,使用ELISA方法检测抗HSV1,抗HSV2,抗CMV和抗EBV的抗体IgG水平。结果与对照组相比,患者组中的抗四种病毒的抗体IgG水平均有较高表达,而抗EBV,抗CMV和抗HSV2,差异有统计学意义(t=2.16,P<0.05;t=4.76,P<0.01;t=3.75,P<0.01)。而在抗HSV1差异无统计学意义(t=0.52,P>0.05)。结论在该研究中,排除遗传学、毒素等其它因素的影响外,EBV,CMV和HSV2病毒可能在天疱疮的发病机理中起作用。HSV1和寻常型天疱疮之间没有观察到有统计学差异,在这方面需要进行更多的研究。

Objective To regard the implication of viruses particularly herpes in pemphigus vulgaris,and assess and compare the Level of immunoglohulin G （lgG） antibodies against herpes simplex virus types 1 and 2 （HSV1 and HSV2）, cytomegalo- virus （CMV） and Epstein-Barr virus （EBV） in patients with pemphigus vulgarisand healthy people. Methods In this study, 23 patients with pemphigus vulgaris and 26 healthy individuals comprised the experimental and control groups, respectively. Serum samples were taken from both groups;the levels of IgG antibodies against HSV1, HSV2 ,CMV and EBV were meas- ured using ELISA. Results Immunoglobulin G titer was higher for all four viruses in the patient group in comparison to the control group. This difference was significant for anti-EBV, anti-CMV and anti-HSV2 （t = 2. 16, P〈0.05 ; t = 4.76, P〈 0.01;t= 3.75, P〈0. 01）, respectively, but not significant for anti HSV1 （t= 0.52, P〉0.05）. Conclusion Viruses including EBV, CMV, and HSV2 probably play a role in the pathogenesis of pemphigus in addition to the effects of genetics,toxins and other predisposing factors. In this study,no statistically significant relationship was observed between HSV1 and pemphigus vulgaris. More studies must he done in this regard.