西安地区健康人群血清NGAL参考值范围的调查

Investigation on the Serum NGAL Reference Range of Healthy Populations in Xi'an

目的建立西安地区健康人群血清中性粒细胞明胶酶相关脂质运载蛋白(NGAL)的参考区间。方法选取2014年3月~2016年10月于陕西省人民医院体检中心健康查体者2 665例(年龄6~95岁,男性1 370例,女性1 295例);来医院预防接种的学龄前儿童682例(年龄0~6岁,男性356例,女性326例)。采用胶乳增强免疫比浊法在BeckmanAU5800全自动生化分析流水线上测定血清NGAL浓度,检测数据进行统计学分析,然后建立西安地区人群不同年龄、性别血清NGAL参考值范围。结果血清NGAL水平在健康人群中呈偏态分布,其中6岁以下学龄前儿童为37.66±23.12 ng/ml,6~15岁为39.25±25.34 ng/ml,16~49岁为46.68±27.06 ng/ml,50~69岁为57.82±29.13 ng/ml,前二者与后者比较差异有统计学意义(t=0.589,P<0.05);而70岁以上为61.87±32.64 ng/ml,15~49岁与70~91岁比较差异有统计学显著性意义(t=8.529,P<0.01)。同时经相关性分析,血清NGAL与年龄较为密切(r=0.298,P<0.01)。而男女之间血清NGAL水平的差异无统计学意义(t=0.263~0.542,均P>0.05)。以50岁为分界年龄,以87.0 ng/ml为参考值上限。结论血清NGAL水平与年龄有关,且随着年龄的增长而呈上升趋势,而与性别无关。

Objective To establish serum NGAL reference range of healthy populations in Xi＇an Area. Methods 2 665 cases （aged 6 to 95 years old,male 1 370, female 1 295） of health-check people were collected from March 2014 to October 2016 in Medical Examination Center of Shaanxi Provincial People＇s Hospital,and 682 cases （aged 0 to 6 years old,male 356 ,female 326） were collected from preschool children of prevention. Serum NGAL concentration of them were analysed by immuno- turbidimetry method with the Automatic Biochemical Analysis Assembly Line of Beckman-AU5800,and the detection data for statistical analysis. Then established the reference range of serum NGAL population of different age and different sex in Xi＇an. Results The serum NGAL levels in healthy subjects showed a skewed distribution,which were preschool children under 6 years of age 37.66±23.12 ng/ml,6-15 years 39.25±25.34 ng/ml,16-49 years 46.68±27.06 ng/ml,and 50- 69 years 57.82± 29.13 ng/ml. Compared the first two with the latter, there was a significant difference （t= 0. 589, P〈 0.05）. The serum NGAL levels of over 70 years were 61.87± 32.64 ng/ml, and there was a significant difference between the ages of 15 and 49 and over 70 years （t=8. 529,P〈0.01）. At the same time,the serum NGAL was closely correlated with age （r=0. 298 ,P±0.01）. But there was no significant difference in serum NGAL level between male and female （t= 0. 263-0. 542,all P〈0.05）. 87ng/ml was the upper limit of the reference value for the age of 50 years. Conclusion The level of serum NGAL was related to age and increased with age,but not with gender.