洋虫改善肺癌荷瘤小鼠体内炎性微环境的研究

Study on Martianus dermestoides Chevrolat improving the inflammatory microenvironment of mice bearing lung cancer

目的探讨洋虫对肺癌荷瘤小鼠体内炎性微环境中IL-6、IL-8和TNF-α的调控情况。方法选用LLC小黑鼠60只,随机分成5组:正常组、肿瘤组、治疗1组、治疗2组、预防组,每组12只。正常组不做处理,正常喂养,共14 d;肿瘤组:单纯接种Lewis肺癌,正常喂养,共14 d;治疗1组:接种Lewis肺癌细胞后,待肿瘤大小长到0.5~1.0 cm后给予常规处理的洋虫(45 mg/kg),共14 d;治疗2组:接种Lewis肺癌细胞后,待肿瘤大小长到0.5~1.0 cm后给予真空冷冻干燥法处理的洋虫(45 mg/kg),共14 d;预防组:接种Lewis肺癌细胞当天给予常规处理的洋虫(45 mg/kg),接种肿瘤后继续洋虫喂养至处死,共14 d。接种肿瘤后第7、14天各组各处死6只C57小鼠,取其血清,ELISA法检测IL-6、IL-8和TNF-α。结果肿瘤组的肿瘤相关炎性因子IL-6、IL-8和TNF-α明显高于正常组,治疗后的肿瘤相关炎性因子IL-6、IL-8和TNF-α明显低于治疗前(P<0.05),治疗1组和治疗2组的肿瘤相关炎性因子表达差异无统计学意义。结论肺癌荷瘤小鼠体内促进肿瘤生长的炎性因子明显升高,中药洋虫可以降低肺癌荷瘤小鼠体内炎性因子的表达。

Objective To investigate the changes of inflammatory microenvironment in which IL-6, IL-8 and TNF-α for mice fed with Martianus dermestoides Chevrolat. Methods 60 male LLC mice were randomly divided into five groups： normal group, tumor group, treatment 1 group, treatment 2 group and prevention group. Each group were 12 rats. The mice was treated with Lewis lung cancer except normal group being not treated. When tumor diameter reach 0.5-1.0 cm, treatment 1 group and treatment 2 group were fed with Martianus dermestoides Chevrolat. The pretreatment group was fed with Martianus dermestoides Chevrolat when treated with Lewis lung cancer. we detected the serum IL-6, IL-8 and TNF-α using ELISA method. Results Tumor associated inflammatory factors IL-6, IL-8, and TNF-α in the tumor group was significantly higher than the normal group, and significantly lower after treatment, the difference was statistically significant（P〈0.05）. Conclusion Inflammatory factors promote lung cancer growth significantly. Martianus dermestoides Chevrolat can regulate inflammatory microenvironment.