摘要
目的对4株昭通乌天麻(Gastrodia elata)共生蜜环菌(Armillaria mella)的生理生化活性进行研究,并鉴定其分类地位。方法通过测定不同碳源和氮源对蜜环菌生长以及胞外酶活性的影响,掌握该蜜环菌生理生化特性,再结合rDNA-IGS序列分析方法进行鉴定。结果不同蜜环菌需要不同的碳源、氮源。3种胞外酶中木聚糖酶活性最大,其次是羧甲基纤维素酶,漆酶活性最小。供试蜜环菌与云南、贵州地区天麻共生蜜环菌以及猪苓共生蜜环菌的亲缘关系比较远。结论蜜环菌能否作为天麻共生菌与其分类地位无关。
Objective To explore the physiological and biochemical activities of 4 strains of Armillaria mella in symbiosis with Zhaotong Gastrodia elata,and its taxonomic status.Methods The influence of different carbon and nitrogen sources on the growth of Armillaria mella as well as the activities of its extracellular enzymes were tested.Identification of the bacteria was performed by using rDNA-IGS sequencing method.Results The optimal carbon source and nitrogen source were different for these strains of Armillaria mella.The activity of xylanase was the highest among three extracellular enzymes,followed by carboxymethyl cellulase,and that of laccase was the lowest.The tested strains of Armillaria mella did not show close genetic relationships with the Armillaria mellastrains in symbiosis with Gastrodia elatain Yunnan and Guizhou regions,neither the Armillaria mella strains in symbiosis with Polyporus umbellatus.Conclusion The taxonomic status of Armillaria mellacan not be used to confirm its symbiotic relationship with Gastrodia elata.
作者
包燚
狄永国
操璟璟
张恒丽
陈丽梅
徐慧妮
李昆志
BAO Yi DI Yongguo CAO Jingjing Zhang Hengli Chen Limei Xu Huini LI Kunzhi(Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan 650500, Chin)
出处
《中国微生态学杂志》
CAS
CSCD
2017年第7期761-765,770,共6页
Chinese Journal of Microecology
基金
国家自然科学基金资助项目(31260297)
昆明理工大学测试基金(20150650)
关键词
天麻
蜜环菌
生理生化特性
鉴定
Gastrodia elata
Armillaria mella
Physiological and biochemical property
Identification