苦参碱对神经胶质瘤U87细胞增殖和侵袭的抑制作用研究

Inhibitory effect of matrine on proliferation and invasion of glioma U87 cells

目的研究苦参碱对神经胶质瘤U87细胞增殖和侵袭的影响,探讨其作用机制。方法采用MTT法和Transwell侵袭实验分别检测苦参碱对U87细胞的增殖能力和侵袭能力。采用Western blotting法和qPCR法检测苦参碱对U87细胞的COX2蛋白和mRNA表达情况。结果药物作用24 h时,与对照组比较,随着苦参碱浓度(5、50、100μg/mL)的增加,苦参碱对U87细胞生长的抑制率不断地增加;相同浓度下,苦参碱对U87细胞增殖率在24、48、72 h时逐渐出现显著性差异(P<0.05)。对照组通过的细胞数量明显多于苦参碱100μg/mL组,两组比较差异具有统计学意义(P<0.01)。与对照组比较,随着苦参碱浓度的增加,COX-2的蛋白和mRNA表达水平明显降低,差异有统计学意义(P<0.05、0.01)。结论苦参碱通过降低COX-2的蛋白和m RNA表达来抑制U87细胞的增殖和侵袭。

Objective To investigate the influence of matrine on glioma U87 proliferation and invasion, and explore its mechanisms. Methods MTT and Transwell were used to detect the influence of matrine on multiplication capacity and invasion ability of U87 cells. Western blotting and qPCR methods were used to detect the COX2 protein and m RNA expression. Results Compared with the control group, inhibition rates of matrine treated for 24 h on the growth rate of U87 cells were increased with the concentrations of matrine（5, 50, 100 g/mL） increased. Under the same concentration, there were significant difference of inhibition rates of matrine treated for 24, 48, and 72 h on the proliferation of U87 cells（P〈0.05）. The number of cells in the control group was significantly more than that in matrine 100 g/mL group, with significant differences between two groups（P〈0.01）. Compared with the control group, the expression of COX-2 protein and mRNA in matrine groups were significantly decreased with the increase of matrine concentration, and the difference was statistically significant（P〈0.05, 0.01）. Conclusion Matrine can inhibit the proliferation and invasion of U87 cells by lowering expression of COX-2 protein and m RNA.