栀子蜂蜜膏外敷治疗兔急性软组织损伤的实验研究

Effect of Gardenia Honey Cream External Application on Acute Soft Tissue Injury in Rabbits

目的:探讨栀子蜂蜜膏对兔急性软组织损伤的影响。方法:34只新西兰大白兔,随机分为正常组4只,实验组10只,扶他林组10只,对照组10只。采取改良击打法制造兔急性软组织损伤模型,造模30min后,实验组外敷栀子蜂蜜膏,扶他林组外用涂抹扶他林,对照组外敷生理盐水纱布,外敷面积略大于击打面积,2次/d.分别于造模后、给药第3天、给药第7天做局部损伤病理组织切片,并记录每天的损伤积分。ELISA法测定各组损伤组织中给药第3天、第7天IL-1及TNF-α含量。各项数据釆用(x±s)表示,用SPSS 18.0软件进行t检验,P<0.05差异有统计学意义。结果:实验组在局部外用栀子蜂蜜膏后,软组织损伤积分低于对照组,差异有统计学意义(P<0.05),并且优于扶他林组;实验组局部外用栀子蜂蜜膏第3天和第7天后,与对照组比较,局部损伤软组织病理形态学有明显改善。在给药第3天和第7天后,实验组兔局部损伤组织内IL-1含量高于正常组,差异有统计学意义(P<0.01,P<0.01),低于对照组(P<0.01,P<0.01),并且低于扶他林组,差异有统计学意义(P<0.05,P<0.05);在给药第3天和第7天后,实验组兔局部损伤软组织内TNF-α含量高于正常组,差异有统计学意义(P<0.01,P<0.01),低于对照组,差异有统计学意义(P<0.01,P<0.01),并且低于扶他林组,差异有统计学意义(P<0.05,P<0.05)。结论:栀子蜂蜜膏可以明显改善兔急性软组织损伤局部症状,可以减少兔局部损伤软组织(肌肉)炎性细胞浸润;栀子蜂蜜膏可以降低兔局部损伤软组织中炎性因子IL-1及TNF-α的含量;栀子蜂蜜膏治疗兔急性软组织损伤的机制可能是通过抑制局部损伤组织中IL-1及TNF-α的表达而起作用。

Objective：To investigate the effect of gardenia honey cream on acute soft tissue injury in rabbits.Methods：Thirty-four New Zealand white rabbits were randomly divided into normal group（n=4）,control group（n=10）,experimental group（n=10）and voltaren treated group（n=10）.The model of acute soft tissue injury of rabbits was made by modified hitting method.After 30 minutes of modeling,the rabbits were applied with different drugs twice a day.The experimental group were treated with gardenia honey cream,voltaren treated group were treated with voltaren,control group was treated with saline,and the applied area was slightly larger than the hit area.The local injury pathological sections were taken respectively after the modeling,day 3 and day 7 after administration.Damage points were record daily.The levels of IL-1 and TNF-αon day 3 and day 7 after administration were determined by ELISA.The data were expressed as mean±standard deviation,with t test with SPSS 18.0 software,P〈0.05 means statistically significant.Results：Damage points in the experimental group was lower than that in the control group（P〈0.05）,and was better than that in the voltaren treated group.Compared with the control group,local injury soft tissue pathology in the experimental group three days and seven days after administration were improved significantly.Compared with normal group,the level of IL-1 in experimental group was higher in three days and seven days after administration（P〈0.01,P〈0.01）;but it was lower than that in voltaren treated group（P〈0.05,P〈0.05）.The level of TNF-αin experimental group was higher in three days and seven days after administration than normal group（P〈0.01,P〈0.01）,and it was lower than that in control group（P〈0.01,P〈0.01）and voltaren treated group（P〈0.05,P〈0.05）.Conclusion：Gardenia honey cream can significantly improve the local symptoms of acute soft tissue injury in rabbits;and it can reduce the infiltration of inflammatory cells in soft tissue（muscle）of rabbits with local damage.It can also reduce the level of inflammatory factors IL-1 and TNF-αin soft tissue of rabbits with local injury.The mechanism of gardenia honey cream in the treatment of acute soft tissue injury in rabbits may be mediated by inhibition the expression of IL-1 and TNF-αin locally injured tissue.