金属阳离子对酿酒酵母J-31发酵影响研究

摘要：本实验通过对酿酒酵母发酵培养基中添加不同浓度梯度的Mg^2＋、Ca^2＋、Fe^2＋、Cu^2＋及不同比例浓度的Fe^2＋和Cu^2＋，测定酿酒酵母的乙醇产量和菌体生物量的变化。结果表明，与空白发酵液相比，四种金属离子均有利于酿酒酵母J-31的生长，并．1iFe2＋和Cu2＋对促进酿酒酵母J-31的生长有显著影响；Mg^2＋和Ca^2＋对促进酿酒酵母J-31发酵产乙醇有显著影响。当Mg^2＋添加浓度为0．1g／L时乙醇产量最大，为30．50g／L；当Ca^2＋添加浓度为1g／L时乙醇产量最大，为28．85g／L。在实验浓度范围内，同时添加Fe^2＋和Cu^2＋，按照0．2：0．1（g／L）的比例添加至培养基，乙醇产量与菌体生长量显著提高，分别为39．95g／L和118．5×10^6个／mL。

This experiment have mensurated the change of ethanol production and biomass of Saccharomyces cerevisiae when added different concentrations of Mg^2＋, Ca^2＋, Fe^2＋, Cu^2＋ and different proportion of concentration of Fe^2＋ and Cu^2＋ to culture medium.The results show that compared with the blank fermentation liquid, four kinds of metal ions were conducive to the growth of Saccharomyces cerevisiae J-31 and the promotability of Fe^2＋ and Cu^2＋ for growth of Saccharomyces cerevisiae J-31 have a significant effect; Mg^2＋ and Ca^2＋ has a significant effect on the fermentation of Saccharomyces cerevisiae J-31 in promoting ethanol production. When the concentration of Mg^2＋ was 0.1 g/L, the ethanol production was the largest as 30.50g/L.when the concentration of Ca^2＋ was 1g/L, the ethanol producton was the largest as 28.85g/L. In the experimental concentration range, while adding Fe^2＋ and Cu^2＋, in accordance to the ratio of 0.2：0.1 （g / L） added to the culture medium, the ethanol yield and biomass have a significantly increasing, respectively as 139.95g/L and 18.5 ×10^6 / mL.