四种分离式培养基检测沙门氏菌的效果比对

Comparison of the effects of four separate culture media for detection of Salmonella

目的:为了更好地开展食品中沙门氏菌的检验工作,使检测工作更加准确、高效,保障食品安全,开展此项目的研究。方法:参照食品国家标准食品微生物检验GB 4789.4-2010食品中沙门氏菌的检验和GB4789.28-2013培养基和试剂的质量要求。结果:本次研究选用的沙门氏菌显色培养基(CAS)、亚硫酸铋琼脂(BS)、木糖赖氨酸脱氧胆盐琼脂(XLD)和HE琼脂(HE)4种培养基的生长率、选择性、特异性均符合GB4789.28-2013中的要求,通过实际加标的结果对比分析发现,显色培养基CAS要优于BS、XLD、HE培养基。结论:在这四种分离式培养基中,显色培养基CAS在分离食品中的沙门氏菌过程中特异性和敏感性是最好。在实际的检测过程中,应灵活运用选择合适的培养基,减少漏检。

Objective： In order to better carry out the inspection work of Salmonella in food, make the detection work more accurate and efficient, to ensure food safety, to carry out the study of this project. Methods： reference to food national standard food microbiological examination GB 4789.4-2010 food testing of Salmonella and GB4789.28-2013 medium and reagent quality requirements. Results： The growth rates of four kinds of culture medium （Salmonella spp.）, Bismuth sulfite （BS）, xylose lysine deoxycholate agar （XLD） and HE agar （HE） were selected. Selectivity and specificity were in accordance with the requirements of GB4789.28-2013. The results showed that the culture medium CAS was better than BS, XLD and HE medium. CONCLUSIONS： In these four separate culture media, the specificity and sensitivity of the chromogenic medium CAS in the isolation of Salmonella in food is the best. In the actual detection process, should be flexible use of the appropriate medium to select, to reduce the leakage test.