猫眼草提取物对乳腺癌2R-75-30细胞抑制作用的研究

The Research of the Inhibition Activity of Euphorbia Lunulata Bge on the 2R-75-30 Breast Cancer Cell

目的:采用体外细胞实验对猫眼草的抗肿瘤活性进行研究,筛选抗肿瘤活性较强的部分,旨在为猫眼草作为抗肿瘤药物的开发提供理论依据。方法:MTT法测定不同浓度的猫眼草不同萃取部分对乳腺癌2R-75-30细胞生长的抑制作用。结果:猫眼草乙酸乙酯、氯仿部分对乳腺癌细胞2R-75-30的生长有抑制作用,且呈现一定的梯度关系,随着药物浓度的升高,对乳腺癌细胞的生长抑制率增强。结论:猫眼草在抗肿瘤方面有一定的研究开发价值。

Objective： The antitumor activity of Euphorbia lunulata Bge was evaluated in vitro cell experiments in order to provide a theoretical basis for the development of cat grass and a new direction and more choices for the development of effective anticancer drugs. Methods： MTT method was used to determine the inhibitory effect of different extraction fractions of Euphorbia lunulata Bge on the growth of breast cancer 2R-75-30 cells. Results： Chloroform layer and ethyl acetate layer of Euphorbia lunulata Bge＇s extracts showed inhibition on the 2R-75-30 breast cancer cell＇s growth and a certain gradient relationship with the drug concentration which increased with the inhibition rate enhancement on the 2R-75-30 breast cancer cell＇s growth. According to the results of the anti-tumor activity measurement, the Euphorbia lunulata Bge＇s extracts in the ethyl acetate layer have anti-tumor activity. Conclusion： This study was designed to provide a theoretical basis to developing Euphorbia lunulata Bge as antioxidant and anticancer drugs.