甲酚皂急性毒性和致突变性试验研究

Experimental study on acute toxicity and mutagenicity of saponated cresol solution

目的为确保甲酚皂消毒液在使用时的安全,对其进行急性毒性和致突变试验研究。方法小鼠急性经口毒性试验和家兔皮肤刺激试验,并以 LD_(50)(3 160 mg/kg)为依据,设计出致突变试验小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸变试验剂量分组,分别为1/2 LD_(50):1 600 mg/kg,1/5 LD_(50):640 mg/kg,1/20 LD_(50):160 mg/kg。结果甲酚皂消毒液对小鼠急性经口 LD_(50)是3 160 mg/kg,属低毒;甲酚皂消毒液原液对家兔皮肤有中等刺激性;小鼠骨髓嗜多染红细胞微核试验和小鼠精子畸变试验甲酚皂各组与阴性对照组比较,差异均无统计学意义(均P>0.05),与阳性对照组(环磷酰胺)比较,差异均有统计学意义(均P<0.01)。结论甲酚皂消毒液急性经口属低毒物,无致突变作用,皮肤刺激试验为中等刺激性。

[Objective]By the experimental study on acute toxicity and mutagenicity of saponated cresol solution,to ensure its security.[Methods]The acute oral toxicity test of mice and dermal irritation test of rabbit were performed. On the basis of LD_（50）（3 160 mg/kg）,the mouse bone marrow polychromatic erythrocyte micronucleus test and mouse sperm aberration test were designed,which the dose groups were as follows：1/2 LD_（50）：1 600 mg/kg,1/5 LD_（50）：640 mg/kg,1/20 LD_（50）：160 mg/kg.[Results]LD_（50）of saponated cresol solution for mice with acute oral toxicity test was 3 160 mg/kg,which belonged to low toxicity. In the dermal irritation test,the solution showed moderate irritation on skins of rabbits. There were not statistically significant differences in the results of mouse bone marrow polychromatic erythrocyte micronucleus test and mouse sperm aberration test between solution dose groups and negative control group（P〉0.05）,while the differences were statistically significant between solution dose groups and positive control group（cyclophosphamide）（P〈0.01）.[Conclusion]The saponated cresol solution belongs to low toxicity in acute oral toxicity test,without mutagenic effect,and shows moderate irritation on skins.